Urinary volatile organic compounds and faecal microbiome profiles in colorectal cancer

Aim Volatile organic compounds (VOCs) are potential biomarkers for diagnosing colorectal cancer (CRC). We characterized urinary VOCs from CRC patients, their spouses/cohabitors (spouses) and first‐degree relatives (relatives) to determine any differences. Correlation with stool‐derived microbiomes w...

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Bibliographic Details
Published in:Colorectal disease 2019-11, Vol.21 (11), p.1259-1269
Main Authors: McFarlane, M., Millard, A., Hall, H., Savage, R., Constantinidou, C., Arasaradnam, R., Nwokolo, C.
Format: Article
Language:English
Subjects:
Cancer
Colorectal cancer
Colorectal carcinoma
environment
genetics
Ionic mobility
Liquid chromatography
microbiome
Microbiomes
Organic compounds
rRNA 16S
Spectrometry
Taxonomy
Technology assessment
Urine
VOCs
Volatile organic compounds
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Summary:Aim Volatile organic compounds (VOCs) are potential biomarkers for diagnosing colorectal cancer (CRC). We characterized urinary VOCs from CRC patients, their spouses/cohabitors (spouses) and first‐degree relatives (relatives) to determine any differences. Correlation with stool‐derived microbiomes was also undertaken. Methods Urine from 56 CRC patients, 45 spouses and 37 relatives was assayed using liquid chromatography, field asymmetric ion mobility spectrometry (FAIMS), mass spectrometer technology. Analysis was performed using five‐fold cross‐validation and a random forest classifier. Faecal microbiome 16S rRNA was sequenced using Illumina MiSeq protocols and analysed using UPARSE and QIIME pipelines. VOC and microbiome profiles were also compared before and after cancer treatment. Results Urinary VOC profiles of CRC patients were indistinguishable from either spouses or relatives. When spouses and relatives were grouped together to form a larger non‐cancer control group (n = 82), their VOC profiles became distinguishable from those of CRC patients (n = 56) with 69% sensitivity and specificity, area under the curve 0.72 (P  1300 operational taxonomic units across all groups. The analysis of similarity R value was 0.067 (P 
ISSN:1462-8910
1463-1318
DOI:10.1111/codi.14739