An Analytical Differential Resistance Pulse System Relying on a Time Shift Signal Analysis–Applications in Coulter Counting

Improving the sensitivity and ultimately the range of particle sizes that can be detected with a single pore extends the versatility of the Coulter counting technique. Here, to enable a pore to have greater sensitivity, we have developed and tested a novel differential resistive pulse sensing (DiS)...

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Bibliographic Details
Published in:ACS sensors 2019-08, Vol.4 (8), p.2190-2195
Main Authors: Birkin, Peter R, Linfield, Steven, Denuault, Guy, Jones, Ronald, Youngs, Jack J, Wain, Emily
Format: Article
Language:English
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Summary:Improving the sensitivity and ultimately the range of particle sizes that can be detected with a single pore extends the versatility of the Coulter counting technique. Here, to enable a pore to have greater sensitivity, we have developed and tested a novel differential resistive pulse sensing (DiS) system for sizing particles. To do this, the response was generated through a time shift approach utilizing a “self-servoing regime” to enable the final signal to operate with a zero background in the absence of particle translocation. The detection and characterization of a series of polystyrene particles, forced to translocate through a cylindrical glass microchannel (GMC) by a suitable static pressure difference using this approach, is demonstrated. An analytical response, which scales with the size of the particles employed, was verified. Parasitic capacitive effects are discussed; however, translocations on the millisecond time scale can be detected with high sensitivity and accuracy using the approach described.
ISSN:2379-3694
2379-3694
DOI:10.1021/acssensors.9b01087