Endophytic fungi from the branches of Camellia taliensis (W. W. Smith) Melchior, a widely distributed wild tea plant

• Published in: World journal of microbiology & biotechnology 2019-07, Vol.35 (7), p.113-15, Article 113
• Main Authors: Chen, Xiaoxue, Luo, Xulu, Fan, Miaomiao, Zeng, Weilin, Yang, Chongren, Wu, Jianrong, Zhao, Changlin, Zhang, Yingjun, Zhao, Ping
• Format: Article
• Language: English
• Subjects: Applied Microbiology; Ascomycota - classification; Ascomycota - genetics; Ascomycota - isolation & purification; Basidiomycota - classification; Basidiomycota - genetics; Basidiomycota - isolation & purification; Biochemistry; Biodiversity; Biomedical and Life Sciences; Biotechnology; Camellia; Camellia - microbiology; China; Cluster Analysis; DNA, Ribosomal - genetics; Endemic plants; Endophytes; Endophytes - classification; Endophytes - genetics; Endophytes - isolation & purification; Environmental Engineering/Biotechnology; Fungi; Fungi - classification; Fungi - genetics; Fungi - isolation & purification; Life Sciences; Microbiology; Myanmar; Organic chemistry; Original Paper; Phylogeny; Sequence Analysis; Spacer; Species richness; Tea
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-019-2686-x

Camellia taliensis (W. W. Smith) Melchior is a wild tea plant endemic from the west and southwest of Yunnan province of China to the north of Myanmar and is used commonly to produce tea by the local people of its growing areas. Its chemical constituents are closely related to those of C. sinensis var. assamica , a widely cultivated tea plant. In this study, the α diversity and phylogeny of endophytic fungi in the branches of C. taliensis were explored for the first time. A total of 160 fungal strains were obtained and grouped into 42 species from 29 genera, which were identified based on rDNA internal transcribed spacer sequence analysis. Diversity analysis showed that the endophytic fungal community of the branches of C. taliensis had high species richness S (42), Margalef index D ′ (8.0785), Shannon–Wiener index H ′ (2.8494), Simpson diversity index D S (0.8891), PIE index (0.8947) and evenness Pielou index J (0.7623) but a low dominant index λ (0.1109). By contrast, that in the branches of C. taliensis had abundant species and high species evenness. Diaporthe tectonigena , Acrocalymma sp. and Colletotrichum magnisporum were the dominant endophytic fungi. The phylogenetic tree was established by maximum parsimony analysis, and the 11 orders observed for endophytic fungi belonging to Ascomycota and Basidiomycota were grouped into 4 classes. Graphic abstract