MYC-driven regulation of long non-coding RNA profiles in breast cancer cells

• Published in: Gene 2019-09, Vol.714, p.143955-143955, Article 143955
• Main Authors: Tokgun, Pervin Elvan, Tokgun, Onur, Kurt, Serap, Tomatir, Ayse Gaye, Akca, Hakan
• Format: Article
• Language: English
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2019.143955

MYC deregulation contributes to breast cancer development and progression. Deregulated expression levels of long non-coding RNAs (lncRNA) have been demonstrated to be critical players in development and/or maintenance of breast cancer. In this study we aimed to evaluate lncRNA expressions depending on MYC overexpression and knockdown in breast cancer cells. Cells were infected with lentiviral vectors by either knockdown or overexpression of c-MYC. LncRNA cDNA was transcribed from total RNA samples and lncRNAs were evaluated by qRT-PCR. Our results indicated that some of the lncRNAs having tumor suppressor (GAS5, MEG3, lincRNA-p21) and oncogenic roles (HOTAIR) are regulated by c-MYC. We observed that c-MYC regulates lncRNAs that have important roles on proliferation, cell cycle and etc. Further studies will give us a light to identify molecular mechanisms related to MYC-lncRNA regulatory pathways in breast cancer. •MYC expressions modulated by lentiviral expression systems we cloned in MCF-7 and MDA-MB-231 cells.•MYC expression regulates the cell cycle related genes in MCF-7 and MDA-MB-231 cells.•Several lncRNAs are being regulated depending on modulated MYC expression in breast cancer cells for the first time.•MYC might regulate the breast cancer progression via lncRNAs.