Phospholipase C-related inactive protein type-1 deficiency affects anesthetic electroencephalogram activity induced by propofol and etomidate in mice

• Published in: Journal of anesthesia 2019-08, Vol.33 (4), p.531-542
• Main Authors: Furukawa, Tomonori, Nikaido, Yoshikazu, Shimoyama, Shuji, Ogata, Yoshiki, Kushikata, Tetsuya, Hirota, Kazuyoshi, Kanematsu, Takashi, Hirata, Masato, Ueno, Shinya
• Format: Article
• Language: English
• Subjects: Adaptor Proteins, Signal Transducing; Analysis; Anesthesia; Anesthesiology; Anesthetics; Anesthetics, General - administration & dosage; Anesthetics, Intravenous - administration & dosage; Animals; Critical Care Medicine; Electroencephalography; Emergency Medicine; Etomidate; Etomidate - administration & dosage; GABA; Intensive; Intracellular Signaling Peptides and Proteins - genetics; Male; Medicine; Medicine & Public Health; Mice; Mice, Inbred C57BL; Mice, Knockout; Neurophysiology; Original Article; Pain Medicine; Phenols; Phospholipases; Propofol - administration & dosage; Receptors, GABA-A - drug effects
• ISSN: 0913-8668; 1438-8359
• DOI: 10.1007/s00540-019-02663-z

Purpose The general anesthetics propofol and etomidate mainly exert their anesthetic actions via GABA A receptor (GABA A -R). The GABA A -R activity is influenced by phospholipase C-related inactive protein type-1 (PRIP-1), which is related to trafficking and subcellular localization of GABA A -R. PRIP-1 deficiency attenuates the behavioral reactions to propofol but not etomidate. However, the effect of these anesthetics and of PRIP-1 deficiency on brain activity of CNS are still unclear. In this study, we examined the effects of propofol and etomidate on the electroencephalogram (EEG). Methods The cortical EEG activity was recorded in wild-type (WT) and PRIP-1 knockout ( PRIP-1 KO ) mice. All recorded EEG data were offline analyzed, and the power spectral density and 95% spectral edge frequency of EEG signals were compared between genotypes before and after injections of anesthetics. Results PRIP-1 deficiency induced increases in EEG absolute powers, but did not markedly change the relative spectral powers during waking and sleep states in the absence of anesthesia. Propofol administration induced increases in low-frequency relative EEG activity and decreases in SEF95 values in WT but not in PRIP-1 KO mice. Following etomidate injection, low-frequency EEG power was increased in both genotype groups. At high frequency, the relative power in PRIP-1 KO mice was smaller than that in WT mice. Conclusions The lack of PRIP-1 disrupted the EEG power distribution, but did not affect the depth of anesthesia after etomidate administration. Our analyses suggest that PRIP-1 is differentially involved in anesthetic EEG activity with the regulation of GABA A -R activity.