Polymorphism association and expression analysis of alpha-lactalbumin (LALBA) gene during lactation in Nili Ravi buffalo

Alpha-lactalbumin has been reported as a highly polymorphic gene that potentially alters the gene expression and is associated with milk composition in dairy breeds. Current study was conducted in two phases. In the first phase, polymorphisms identification in alpha-lactalbumin (LALBA) gene and its...

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Bibliographic Details
Published in:Tropical animal health and production 2020, Vol.52 (1), p.265-271
Main Authors: Manzoor, Sidra, Nadeem, Asif, Javed, Maryam
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Buffalo
Cattle
Composition
Deoxyribonucleic acid
DNA
Gene expression
Gene polymorphism
Gene regulation
Lactalbumin
Lactation
Life Sciences
Livestock
Livestock farming
Mastitis
Milk
Milk production
Nucleotide sequence
Phase transitions
Polymorphism
Regular Articles
Veterinary Medicine/Veterinary Science
Whey
Whey protein
Zoology
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Summary:Alpha-lactalbumin has been reported as a highly polymorphic gene that potentially alters the gene expression and is associated with milk composition in dairy breeds. Current study was conducted in two phases. In the first phase, polymorphisms identification in alpha-lactalbumin (LALBA) gene and its association with milk composition was performed. To identify the genetic polymorphism, Nili Ravi buffaloes at their second lactation were selected from Government livestock farm (Buffalo Research Institute, Pattoki). Genomic DNA was extracted from blood samples. After PCR amplification, products were sequenced, and data was analyzed. Results showed that the identified polymorphisms at chromosomal position 34310940 were found associated with major whey protein. In the second phase of study, milk samples were collected from five healthy mastitis-free Nili Ravi buffaloes in their second lactation for expression analysis of alpha-lactalbumin gene at their transition (day 15), mid (day 90), and late (day 250) lactation. Gene expression was observed highest in transition phase with a gradual decrease of expression in mid and late phase of lactation. Further studies are needed to explore the regulation of milk production genes and their translational efficiency during the course of lactation.
ISSN:0049-4747
1573-7438
DOI:10.1007/s11250-019-02010-0