HbA1c adjusted by erythrocyte creatine is a useful glycemic control indicator in patients with hemolysis

• Published in: Clinical biochemistry 2019-11, Vol.73, p.77-81
• Main Authors: Koga, Masafumi, Inada, Shinya, Shibata, Masaru, Ijima, Hiroko, Jinnouchi, Hideaki, Ono, Yasuhiro, Iwasaka, Tsuyoshi, Tokuhiro, Shinji, Matsumura, Yoshihisa, Matsui, Hirotaka, Okumiya, Toshika
• Format: Article
• Language: English
• Subjects: Erythrocyte creatine; Glycated albumin; HbA1c; Hemolysis
• ISSN: 0009-9120; 1873-2933
• DOI: 10.1016/j.clinbiochem.2019.08.004

HbA1c shows low in patients with hemolysis, whereas glycated albumin (GA) is not affected by hemolysis. Therefore, the GA/HbA1c ratio reflects hemolysis in diabetic patients with hemolysis. Erythrocyte creatine (EC) is an indicator of hemolysis that reflects the mean erythrocyte age. The aim of this study was to examine whether HbA1c adjusted by EC accurately reflected glycemic control in patients with hemolysis. A total of 238 individuals, consisting of 131 diabetic patients and 107 non-diabetic subjects, and consisting of 42 patients with hemolysis, and 196 subjects without hemolysis were selected for the study. HbA1c expressed in the IFCC units (iA1c) as well as in the NGSP units (A1C) were used. From the fact that EC and the GA/iA1c ratio showed a significant positive correlation, a formula for iA1c adjusted by EC (ECadj-iA1c) was created from a regression equation between EC and the GA/iA1c ratio. Significant correlations were observed between the GA/iA1c ratio and various hemolytic indicators but not between the GA/ECadj-iA1c ratio and those hemolytic indicators. The GA/iA1c ratio in individuals with hemolysis was significantly higher than in individuals without hemolysis, while no significant differences were observed in the GA/ECadj-iA1c ratio between the groups. Further, iA1c concentrations in non-diabetic patients with hemolysis were significantly lower than in the non-diabetic subjects without hemolysis, whereas ECadj-iA1c and GA concentrations showed no significant difference between the two groups. These results suggested that ECadj-iA1c accurately reflected glycemic control in patients with hemolysis. •HbA1c, but not glycated albumin (GA), show low in patients with hemolysis.•Erythrocyte creatine (EC) is a hemolytic indicator that reflects the erythrocyte age.•We created a formula for IFCC-HbA1c (iA1c) adjusted with EC (ECadj-iA1c).•GA/ECadj-iA1c ratio was not significantly increased with the degree of hemolysis.•ECadj-iA1c might accurately reflect glycemic control in patients with hemolysis.