Acyl-CoA: lysophosphatidylcholine acyltransferases (LPCATs) of Camelina sativa seeds: biochemical properties and function

The transfer of polyunsaturated fatty acids from the location of their synthesis (phosphatidylcholine) to other lipids, e.g., triacylglycerol, remains insufficiently understood. Several enzymes could be involved in this process. One of these enzymes is acyl-CoA:lysophosphatidylcholine acyltransferas...

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Bibliographic Details
Published in:Planta 2019-11, Vol.250 (5), p.1655-1670
Main Authors: Klińska, Sylwia, Jasieniecka-Gazarkiewicz, Katarzyna, Banaś, Antoni
Format: Article
Language:English
Subjects:
1-Acylglycerophosphocholine O-Acyltransferase - genetics
1-Acylglycerophosphocholine O-Acyltransferase - metabolism
Agriculture
Biomedical and Life Sciences
Brassicaceae - enzymology
Brassicaceae - genetics
Brassicaceae - growth & development
Calcium
Camelina sativa
Developmental stages
Ecology
Enzymes
Fatty acids
Forestry
Lecithin
Life Sciences
Lipids
Lysophosphatidylcholine
Magnesium
ORIGINAL ARTICLE
pH effects
Phosphatidylcholine
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Sciences
Polyunsaturated fatty acids
Seeds
Seeds - enzymology
Seeds - genetics
Seeds - growth & development
Triglycerides
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Summary:The transfer of polyunsaturated fatty acids from the location of their synthesis (phosphatidylcholine) to other lipids, e.g., triacylglycerol, remains insufficiently understood. Several enzymes could be involved in this process. One of these enzymes is acyl-CoA:lysophosphatidylcholine acyltransferases (LPCATs). In Camelina sativa seeds, LPCATs could be one of the most important players in this process. Our data clearly indicate that the CsLPCATs present in developing seeds have the potential to transfer almost all polyunsaturated fatty acids synthesised on phosphatidylcholine to the acyl-CoA pool. CsLPCAT activity is the highest at 30°C, and the enzymes operate well at a pH of 7.0–11.0, with the best activity at a pH of 9.0. The activity of CsLPCATs was inhibited by calcium and magnesium ions at a concentration of 0.05–2 mM. In the forward reaction, CsLPCATs preferentially utilise 18:2-CoA; however, other C18 unsaturated fatty acids are also well accepted. In the backward reactions, there is no clear discrimination between the C18 unsaturated fatty acids utilised by the enzymes for phosphatidylcholine remodelling. The activity of CsLPCATs does not differ much between the stages of seed development.
ISSN:0032-0935
1432-2048
DOI:10.1007/s00425-019-03248-6