Changes in antibiotic resistance genes and mobile genetic elements during cattle manure composting after inoculation with Bacillus subtilis

[Display omitted] •sul1 mainly be responsible for changes in ARGs during composting.•Adding Bacillus subtilis at 0.5% reduced relative abundances of ARGs and MGEs.•Pathogenic bacteria were largely eliminated in compost with Bacillus subtilis.•intI1, Firmicutes, and pH were the main drivers of variat...

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Bibliographic Details
Published in:Bioresource technology 2019-11, Vol.292, p.122011-122011, Article 122011
Main Authors: Duan, Manli, Zhang, Yuhua, Zhou, Beibei, Wang, Quanjiu, Gu, Jie, Liu, Guohuan, Qin, Zhenlun, Li, Zhijian
Format: Article
Language:English
Subjects:
Antibiotic resistance gene
Bacillus subtilis
Bacterial community
Composting
intI1
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Summary:[Display omitted] •sul1 mainly be responsible for changes in ARGs during composting.•Adding Bacillus subtilis at 0.5% reduced relative abundances of ARGs and MGEs.•Pathogenic bacteria were largely eliminated in compost with Bacillus subtilis.•intI1, Firmicutes, and pH were the main drivers of variations in ARGs. This study explored the effects of Bacillus subtilis at four levels (0, 0.5%, 1%, and 2% w/w compost) on the variations in ARGs, mobile genetic elements (MGEs), and the bacterial community during composting. The composting process had a greater impact on ARGs than Bacillus subtilis. The main ARG detected was sul1. The addition of Bacillus subtilis at 0.5% reduced the relative abundances of ARGs, MGEs, and human pathogenic bacteria (by 2–3 logs) in the mature products. Network and redundancy analyses suggested that intI1, Firmicutes, and pH were mainly responsible for the changes in ARGs, thus controlling these factors might help to inhibit the spread of ARGs.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2019.122011