Differential expression of recently duplicated PTOX genes in Glycine max during plant development and stress conditions

• Published in: Journal of bioenergetics and biomembranes 2019-10, Vol.51 (5), p.355-370
• Main Authors: Maia, Rachel Alves, da Cruz Saraiva, Kátia Daniella, Roque, André Luiz Maia, Thiers, Karine Leitão Lima, dos Santos, Clesivan Pereira, da Silva, João Hermínio Martins, Feijó, Daniel Ferreira, Arnholdt-Schmitt, Birgit, Costa, José Hélio
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Apoptosis; Biochemistry; Biological evolution; Bioorganic Chemistry; Biosynthesis; Chemistry; Chemistry and Materials Science; Chloroplast Proteins - genetics; Chloroplasts; Environmental stress; Gene duplication; Gene expression; Gene Expression Regulation, Plant; Genes; Genes, Plant - genetics; Genomes; Glycine max; Glycine max - genetics; Glycine max - growth & development; Histology; Metabolism; Molecular docking; Molecular Docking Simulation; Morphology; Organic Chemistry; Oxidation; Oxidoreductases - genetics; Oxidoreductases - metabolism; Oxygen; Phylogeny; Plant Development - genetics; Plant metabolism; Plant Proteins - genetics; Plant species; Plants (botany); Plastid terminal oxidase; Plastids - enzymology; Plastoquinol; Plastoquinone - analogs & derivatives; Plastoquinone - metabolism; Proteins; Reproduction (copying); RNA, Messenger - metabolism; Senescence; Speciation; Stress, Physiological - genetics; Stresses; Substrates; Terminal oxidase
• ISSN: 0145-479X; 1573-6881
• DOI: 10.1007/s10863-019-09810-x

Plastid terminal oxidase (PTOX) is a chloroplast enzyme that catalyzes oxidation of plastoquinol (PQH 2 ) and reduction of molecular oxygen to water. Its function has been associated with carotenoid biosynthesis, chlororespiration and environmental stress responses in plants. In the majority of plant species, a single gene encodes the protein and little is known about events of PTOX gene duplication and their implication to plant metabolism. Previously, two putative PTOX ( PTOX1 and 2 ) genes were identified in Glycine max , but the evolutionary origin and the specific function of each gene was not explored. Phylogenetic analyses revealed that this gene duplication occurred apparently during speciation involving the Glycine genus ancestor, an event absent in all other available plant leguminous genomes. Gene expression evaluated by RT-qPCR and RNA-seq data revealed that both PTOX genes are ubiquitously expressed in G. max tissues, but their mRNA levels varied during development and stress conditions. In development, PTOX1 was predominant in young tissues, while PTOX2 was more expressed in aged tissues. Under stress conditions, the PTOX transcripts varied according to stress severity, i.e., PTOX1 mRNA was prevalent under mild or moderate stresses while PTOX2 was predominant in drastic stresses. Despite the high identity between proteins (97%), molecular docking revealed that PTOX1 has higher affinity to substrate plastoquinol than PTOX2. Overall, our results indicate a functional relevance of this gene duplication in G. max metabolism, whereas PTOX1 could be associated with chloroplast effectiveness and PTOX2 to senescence and/or apoptosis.