First Molecular Evidences of Acanthamoeba T3, T4 and T5 Genotypes in Hemodialysis Units in Iran

Background Acanthamoeba is a genus of the free-living amoeba that is widespread in the environment and is a causative agent of opportunistic infections in human. This study aimed to investigate the existence and genotyping of Acanthamoeba species in hemodialysis units in Iran. Methods In the present...

Full description

Saved in:
Bibliographic Details
Published in:Acta parasitologica 2019-12, Vol.64 (4), p.911-915
Main Authors: Saberi, Reza, Fakhar, Mahdi, Sedighi, Omid, Espahbodi, Fatemeh, Latifi, Alireza, Makhlough, Atieh, Sharifpour, Ali, Ziaei Hezarjaribi, Hajar, Asfaram, Shabnam
Format: Article
Language:English
Subjects:
Acanthamoeba
Acanthamoeba - genetics
Acanthamoeba - isolation & purification
Acanthamoeba - pathogenicity
Amoeba
Animal Systematics/Taxonomy/Biogeography
Biofilms
Biomedical and Life Sciences
Biomedicine
Contamination
Dialysis
DNA, Protozoan - genetics
Dust
Dust - analysis
Dust control
Ecology
Genotype
Genotypes
Genotyping
Hemodialysis
Hemodialysis Units, Hospital
Hydraulic equipment
Infections
Iran
Medical Microbiology
Microbiology
Morphology
Original Paper
Parasitology
Renal Dialysis
RNA, Ribosomal, 18S - genetics
rRNA 18S
Sequence analysis
Sequence Analysis, DNA
Water - parasitology
Water analysis
Water sampling
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background Acanthamoeba is a genus of the free-living amoeba that is widespread in the environment and is a causative agent of opportunistic infections in human. This study aimed to investigate the existence and genotyping of Acanthamoeba species in hemodialysis units in Iran. Methods In the present study, forty water samples of hydraulic systems and twenty dust samples were collected from two hemodialysis units in Mazandaran Province, northern Iran. The samples were cultivated on non-nutrient agar and genotyping was performed by targeting the 18S rRNA gene. Results Both morphology and molecular analyses showed that 17.5% (7/40) of water samples and 50% (10/20) of dust samples were positive for Acanthamoeba spp. The sequencing analysis of these isolates was found to be T3, T4 and T5 genotypes. Discussion To the best of our knowledge, this is the first investigation to identify of Acanthamoeba species in hydraulic system of hemodialysis units in Iran. High contamination of hemodialysis units with virulent T4 genotype of Acanthamoeba may poses a risk for biofilm formation. Our results support urgent need to improve filtration methods in dialysis units and monitoring hemodialysis patients for Acanthamoeba infections.
ISSN:1230-2821
1896-1851
DOI:10.2478/s11686-019-00122-z