Exploring the molecular mechanism of the effect of puerarin on P2X3

• Published in: International journal of biological macromolecules 2020-01, Vol.142, p.484-491
• Main Authors: Liu, Shuangmei, Wang, Mengke, Wang, Na, Li, Shizhen, Sun, Rui, Xing, Jingming, Wang, Yueying, Yu, Shicheng, Li, Lin, Li, Guodong, Liang, Shangdong
• Format: Article
• Language: English
• Subjects: P2X3; Puerarin; Site-directed mutagenesis
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2019.09.120

•Molecular docking revealed puerarin binds well to hP2X3 protein.•Puerarin interacts with hP2X3 protein near ATP-binding pocket.•V64A mutation reduces the effects of the P2X3 receptor and puerarin.•Puerarin inhibits P2X3 receptor by binding to V64A. P2X3 is a ligand-gated nonselective cation channel and permeable to Na+, K+ and Ca2+. Adenosine triphosphate (ATP) activation of the P2X3 on primary sensory ganglion neurons is involved in nociceptive transmission. Puerarin is a major active ingredient extracted from the traditional Chinese medicine Ge-gen. Puerarin inhibits nociceptive signal transmission by inhibiting the P2X3 in the dorsal root ganglia (DRG) and sympathetic ganglia, but its molecular mechanism is unclear. The aim of this study was to explore the molecular mechanism of puerarin on the P2X3. Here, molecular docking results revealed that puerarin binds well to the human P2X3 protein in the vicinity of the ATP binding pocket. Protein-ligand docking showed that the V64A mutation reduced the effect of puerarin but had little effect on ATP. V64A site-directed mutagenesis of P2X3 was performed using an overlap extension PCR technique. The wild-type and V64A mutant pEGFP-C1-P2X3 recombinant plasmids were transfected into HEK 293 cells. The electrophysiology results demonstrated that puerarin exerted an obvious inhibitory effect on ATP-activated currents in HEK 293 cells transfected with the wild-type P2X3, while little inhibition was observed in HEK 293 cells transfected with the mutant P2X3. These studies suggest that puerarin inhibits the P2X3 by binding to V64A.