Exposure to Waterborne nTiO2 Reduces Fertilization Success and Increases Polyspermy in a Bivalve Mollusc: A Threat to Population Recruitment

Fertilization success is crucial for the population recruitment of an organism. However, little is known about the threat of nanoparticles (NPs) to the fertilization of broadcast spawners. Therefore, the effects of nTiO2 on fertilization success, polyspermy rate, sperm velocity, gametic DNA damage,...

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Bibliographic Details
Published in:Environmental science & technology 2019-11, Vol.53 (21), p.12754-12763
Main Authors: Han, Yu, Shi, Wei, Rong, Jiahuan, Zha, Shanjie, Guan, Xiaofan, Sun, Hongxiang, Liu, Guangxu
Format: Article
Language:English
Subjects:
Collision dynamics
Damage
Deoxyribonucleic acid
DNA
DNA damage
Exposure
Fertilization
Gametes
Gametocytes
Invertebrates
Microvillus
Mollusks
Nanoparticles
Recruitment
Spawning
Sperm
Success
Swimming
Ultrastructure
Velocity
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Summary:Fertilization success is crucial for the population recruitment of an organism. However, little is known about the threat of nanoparticles (NPs) to the fertilization of broadcast spawners. Therefore, the effects of nTiO2 on fertilization success, polyspermy rate, sperm velocity, gametic DNA damage, sperm–egg collision probability, gamete fusion, and oocyte ultrastructure were investigated in a broadcast spawning bivalve, Tegillarca granosa. The results obtained show that fertilization success significantly decreased, whereas polyspermy risk markedly increased upon nTiO2 exposure. In addition, nTiO2 exposure led to a significant reduction in sperm swimming velocity, which would subsequently constrain gamete collisions. In addition, nTiO2 exposure resulted in a significant decline in gamete fusion per collision along with aggravated DNA damage in gametes. Furthermore, ultrastructural analysis illustrated the attachment of nTiO2 to the oocyte surface, which subsequently resulted in microvillus disassociation and plasma membrane damage. In conclusion, the results obtained suggest a significant threat from NP pollution to the recruitment of broadcast spawning invertebrates.
ISSN:0013-936X
1520-5851
DOI:10.1021/acs.est.9b03675