The testis-specific expressed gene Spata34 is not required for fertility in mice

It is estimated that more than two thousand genes exhibit testis-predominant expression pattern. The functions of hundreds of these genes have been explored during mouse spermatogenesis. However, there are still many genes whose relevance to reproduction in vivo remains unexplored. Our previous stud...

Full description

Saved in:
Bibliographic Details
Published in:Molecular biology reports 2020, Vol.47 (1), p.285-292
Main Authors: Nie, DongSong, Dai, YanFa, Luo, ZhongQin
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Animals
Base Sequence
Biomedical and Life Sciences
Body weight
Cell cycle
CRISPR
Fertility
Fertility - genetics
Gene Expression
Genes
Genome editing
Genomes
Histology
Life Sciences
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Morphology
Original Article
Repressor Proteins - genetics
Repressor Proteins - metabolism
Sperm Count
Spermatogenesis
Spermatogenesis - genetics
Spermatozoa - metabolism
Spermatozoa - physiology
Testes
Testis - physiology
Transcriptome
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:It is estimated that more than two thousand genes exhibit testis-predominant expression pattern. The functions of hundreds of these genes have been explored during mouse spermatogenesis. However, there are still many genes whose relevance to reproduction in vivo remains unexplored. Our previous studies, as well as the other documented study, have indicated that Spata34 , an evolutionarily conserved gene in metazoan species, was exclusively expressed in mouse testes and involved in spermatogenesis by regulating cell cycle progression. The present study aims to determine the effect of Spata34 gene knockout on mouse reproduction in vivo by generating a Spata34 gene knockout model using CRISPR/Cas9-mediated genome editing technology. We found that the Spata34 gene KO mice had normal fertility compared with wild type mice, and no overt detectable difference was found in testis/body weight ratios, testicular histology, sperm counts and spermatozoa motility parameters between WT and Spata34 KO mice. Our report indicated that the testis-specific-expressed gene Spata34 was not required for male mouse fertility, which will help to avoid unnecessary expenditures and effort by other researchers.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-019-05131-9