Quantification of fenoprofen in human plasma using UHPLC–tandem mass spectrometry for pharmacokinetic study in healthy subjects

A rapid, simple and sensitive ultra‐performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method has been developed to quantify fenoprofen, a nonsteroidal anti‐inflammatory drug in human plasma for a pharmacokinetic study in healthy subjects. Owing to high levels of protein bindin...

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Bibliographic Details
Published in:Biomedical chromatography 2020-01, Vol.34 (1), p.e4708-n/a
Main Authors: Bharwad, Kirtikumar D., Shah, Priyanka A., Shrivastav, Pranav S., Sharma, Vinay S., Singhal, Puran
Format: Article
Language:English
Subjects:
Adult
Chromatography, High Pressure Liquid - methods
Drug Stability
fenoprofen
Fenoprofen - blood
Fenoprofen - chemistry
Fenoprofen - pharmacokinetics
fenoprofen‐d3
human plasma
Humans
Linear Models
Male
Middle Aged
pharmacokinetic study
Reproducibility of Results
Sensitivity and Specificity
solid‐phase extraction
Tandem Mass Spectrometry - methods
UHPLC–MS/MS
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Summary:A rapid, simple and sensitive ultra‐performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method has been developed to quantify fenoprofen, a nonsteroidal anti‐inflammatory drug in human plasma for a pharmacokinetic study in healthy subjects. Owing to high levels of protein binding, protein precipitation followed by solid‐phase extraction was employed for the extraction of fenoprofen and fenoprofen‐d3 (used as internal standard) from 200 μL human plasma. Separation was performed on a BEH C18 (50 × 2.1 mm, 1.7 μm) column using methanol−0.2% acetic acid in water (75:25, v/v) under isocratic elution. Electrospray ionization was operated in the negative mode for sample ionization. Ion transitions used for quantification in the selected reaction monitoring mode were m/z 241/197 and m/z 244/200 for fenoprofen and fenoprofen‐d3, respectively. Under the optimized conditions, fenoprofen showed excellent linearity in the concentration range 0.02–20 μg/mL (r2 ≥ 0.9996), adequate sensitivity, favorable accuracy (96.4–103.7%) and precision (percentage coefficient of variation ≤4.3) with negligible matrix effect. The validated method was successfully applied to a pharmacokinetic study of fenoprofen in healthy subjects. The significant features of the method include higher sensitivity, small plasma volume for processing and a short analysis time.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.4708