Reliable Methods for Analyses of Volatile Compounds of Copaifera Oleoresins Combining Headspace and Gas Chromatography

Two analytical methods were developed in this study for direct and fast chemical investigation of authentic Copaifera oleoresins (COR) and commercial products. Polydimethylsiloxane microfiber coupled to gas chromatography‐mass spectrometry (HS‐SPME‐GC/MS) showed the best results for oleoresin qualit...

Full description

Saved in:
Bibliographic Details
Published in:Chemistry & biodiversity 2020-01, Vol.17 (1), p.e1900440-n/a
Main Authors: Mangabeira da Silva, Jonas Joaquim, Pena Ribeiro, Victor, Lemos, Marivane, Miller Crotti, Antônio Eduardo, Rogez, Hervé, Kenupp Bastos, Jairo
Format: Article
Language:English
Subjects:
Analytical methods
Caryophyllene
Chemical composition
Chromatography
Copaifera
Copaifera oleoresins
Fabaceae - chemistry
Flame ionization detectors
Gas chromatography
Gas Chromatography-Mass Spectrometry
Headspace
Ionization
Mass spectrometry
Mass spectroscopy
Microfibers
Oleoresins
Organic chemistry
Polydimethylsiloxane
Qualitative analysis
Quantitative analysis
Sesquiterpenes
Solid phase methods
Solid Phase Microextraction
Volatile compounds
Volatile Organic Compounds - analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two analytical methods were developed in this study for direct and fast chemical investigation of authentic Copaifera oleoresins (COR) and commercial products. Polydimethylsiloxane microfiber coupled to gas chromatography‐mass spectrometry (HS‐SPME‐GC/MS) showed the best results for oleoresin qualitative analysis, setting the following extraction conditions: equilibrium time of 15 min, extraction time of 30 min, extraction temperature at 60 °C and constant stirring of 400 rpm. Sesquiterpenes α‐copaene, β‐elemene, β‐caryophyllene and trans‐α‐bergamotene were found in all investigated samples. Quantitative analysis by gas chromatography coupled with flame ionization detector (GC‐FID) measured the content of the four sesquiterpenes in all samples. Qualitative and quantitative results showed important differences between COR of distinct species and commercial products. Data regarding the volatile composition of C. oblongifolia and C. trapezifolia oleoresins were first presented in this study and two new analytical methods were reported for direct and fast qualitative and quantitative analysis of COR.
ISSN:1612-1872
1612-1880
DOI:10.1002/cbdv.201900440