Using microbial seed coating for improving cowpea productivity under a low‐input agricultural system

• Published in: Journal of the science of food and agriculture 2020-02, Vol.100 (3), p.1092-1098
• Main Authors: Rocha, Inês, Souza‐Alonso, Pablo, Pereira, Graça, Ma, Ying, Vosátka, Miroslav, Freitas, Helena, Oliveira, Rui S.
• Format: Article
• Language: English
• Subjects: Agricultural production; agricultural sustainability; arbuscular mycorrhizal fungi; Arbuscular mycorrhizas; Coating; Coatings; Cowpeas; Crop Production - methods; Crop yield; field experiment; Fungi; Glomeromycota - physiology; Grain; Inoculation; Lipids; Microorganisms; Mycorrhizae - physiology; Nutrient content; Organic chemistry; Organic matter; Organic soils; plant growth promoting rhizobacteria; Productivity; Pseudomonas - physiology; Pseudomonas libanensis; Rhizophagus irregularis; Seed coats; Seeds; Seeds - growth & development; Seeds - microbiology; Soil - chemistry; Soil organic matter; Vigna - growth & development; Vigna - microbiology; Vigna unguiculata
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.10117

BACKGROUND Plant‐growth‐promoting rhizobacteria (PGPR) and arbuscular mycorrhizal (AM) fungi have the ability to enhance the growth, fitness, and quality of various agricultural crops, including cowpea. However, field trials confirming the benefits of microbes in large‐scale applications using economically viable and efficient inoculation methods are still scarce. Microbial seed coating has a great potential for large‐scale agriculture through the application of reduced amounts of PGPR and AM fungi inocula. Thus, in this study, the impact of seed coating with PGPR, Pseudomonas libanensis TR1 and AM fungus, Rhizophagus irregularis (single or multiple isolates) on grain yield and nutrient content of cowpea under low‐input field conditions was evaluated. RESULTS Seed coating with P. libanensis + multiple isolates of R. irregularis (coatPMR) resulted in significant increases in shoot dry weight (76%), and in the number of pods and seeds per plant (52% and 56%, respectively) and grain yield (56%), when compared with non‐inoculated control plants. However, seed coating with P. libanensis + R. irregularis single‐isolate (coatPR) did not influence cowpea grain yield. Grain lipid content was significantly higher (25%) in coatPMR plants in comparison with control. Higher soil organic matter and lower pH were observed in the coatPMR treatment. CONCLUSIONS Our findings indicate that cowpea field productivity can be improved by seed coating with PGPR and multiple AM fungal isolates under low‐input agricultural systems. © 2019 Society of Chemical Industry