Peptide-induced super-assembly of biocatalytic metal-organic frameworks for programmed enzyme cascades

Despite the promise of metal-organic frameworks (MOFs) as functional matrices for enzyme stabilization, the development of a stimulus-responsive approach to induce a multi-enzyme cascade reaction in MOFs remains a critical challenge. Here, a novel method using peptide-induced super-assembly of MOFs...

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Bibliographic Details
Published in:Chemical science (Cambridge) 2019-09, Vol.1 (34), p.7852-7858
Main Authors: Liang, Jieying, Mazur, Federico, Tang, Chuyang, Ning, Xunan, Chandrawati, Rona, Liang, Kang
Format: Article
Language:English
Subjects:
Cascade chemical reactions
Catalysis
Catalytic activity
Coils
Dismantling
Enzymes
Metal-organic frameworks
Nanoparticles
Organic chemistry
Peptides
Smart materials
Superstructures
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Summary:Despite the promise of metal-organic frameworks (MOFs) as functional matrices for enzyme stabilization, the development of a stimulus-responsive approach to induce a multi-enzyme cascade reaction in MOFs remains a critical challenge. Here, a novel method using peptide-induced super-assembly of MOFs is developed for programmed enzyme cascade reactions on demand. The super-assembled MOF particles containing different enzymes show remarkable 7.3-fold and 4.4-fold catalytic activity enhancements for the two-enzyme and three-enzyme cascade reactions, respectively, as compared with the unassembled MOF nanoparticles. Further digestion of the coiled-coil forming peptides on the MOF surfaces leads to the MOF superstructure disassembly and the programmed enzyme cascade reaction being "switched-off". Research on these stimuli-responsive materials with controllable and predictable biocatalytic functions/properties provide a concept to facilitate the fabrication of next-generation smart materials based on precision chemistry. Complementary peptide-induced super-assembly of biocatalytic MOFs is developed for programmed enzyme cascades on demand.
ISSN:2041-6520
2041-6539
DOI:10.1039/c9sc02021g