Characterization and immunogenicity of bone marrow-derived mesenchymal stem cells under osteoporotic conditions

Mesenchymal stem cells (MSCs) are characterized by their multilineage potential and low immunogenicity. However, the properties of MSCs under pathological conditions are unclear. The current study investigated the differentiation potential and immunological characteristics of bone marrow-derived MSC...

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Bibliographic Details
Published in:Science China. Life sciences 2020-03, Vol.63 (3), p.429-442
Main Authors: Huang, Yingkang, Yin, Yin, Gu, Yanzheng, Gu, Qiaoli, Yang, Huilin, Zhou, Zhengyu, Shi, Qin
Format: Article
Language:English
Subjects:
Adipogenesis
Apoptosis
Biomedical and Life Sciences
Bone loss
Bone marrow
CD3 antigen
CD40 antigen
CD80 antigen
Cell death
Cell proliferation
Chondrogenesis
Cytokines
Cytology
Immunogenicity
Inflammation
Life Sciences
Lymphocytes T
Mesenchymal stem cells
Osteocalcin
Osteogenesis
Osteoporosis
Ovariectomy
Research Paper
Stem cell transplantation
Stem cells
Surface markers
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Summary:Mesenchymal stem cells (MSCs) are characterized by their multilineage potential and low immunogenicity. However, the properties of MSCs under pathological conditions are unclear. The current study investigated the differentiation potential and immunological characteristics of bone marrow-derived MSCs from ovariectomized-osteoporotic rats (OP-BMSCs). Although the expression of cell morphology- and stemness-related surface markers was similar between OP-BMSCs and BMSCs from healthy rats (H-BMSCs), the proliferation rate was significantly decreased compared with that of H-BMSCs. Regarding multilineage potential, osteogenesis and chondrogenesis abilities of OP-BMSCs decreased, but the adipogenesis ability was significantly enhanced compared with that of H-BMSCs. As expected, decreased osteogenesis following osteogenic induction resulted in reduced expression of β-catenin, osteocalcin, and runt-related transcription factor 2 in OP-BMSCs. Remarkably, the expression of the co-stimulatory proteins CD40 and CD80 was significantly higher, whereas the expression of the negative co-stimulatory molecule programmed cell death ligand 1 was significantly lower in the OP-BMSCs than that in H-BMSCs. Consequently, H-BMSCs inhibited the proliferation and secretion of inflammatory cytokines from anti-CD3 antibody-activated T cells, whereas OP-BMSCs did not. These results indicate that decreased osteogenesis and increased immunogenicity of OP-BMSCs contribute to bone loss in osteoporosis.
ISSN:1674-7305
1869-1889
DOI:10.1007/s11427-019-1555-9