An Inactive Dispersin B Probe for Monitoring PNAG Production in Biofilm Formation

• Published in: ACS chemical biology 2020-05, Vol.15 (5), p.1204-1211
• Main Authors: Eddenden, Alexander, Kitova, Elena N, Klassen, John S, Nitz, Mark
• Format: Article
• Language: English
• Subjects: Aggregatibacter actinomycetemcomitans - metabolism; Bacterial Proteins - chemistry; Bacterial Proteins - metabolism; beta-Glucans - chemistry; Biofilms; Catalytic Domain; Cell Aggregation; Cell Membrane - metabolism; Cell Membrane - ultrastructure; Escherichia coli - physiology; Fluorescent Dyes - chemistry; Fluorescent Dyes - metabolism; Glycoside Hydrolases - chemistry; Glycoside Hydrolases - metabolism; Green Fluorescent Proteins - chemistry; Mutation; Optical Imaging; Recombinant Proteins - chemistry; Recombinant Proteins - metabolism; Single-Cell Analysis
• ISSN: 1554-8929; 1554-8937
• DOI: 10.1021/acschembio.9b00907

The bacterial exopolysaccharide poly-β-1,6-N-acetylglucosamine is a major extracellular matrix component in biofilms of both Gram-positive and Gram-negative organisms. We have leveraged the specificity of the biofilm-dispersing glycoside hydrolase Dispersin B (DspB) to generate a probe (Dispersin B PNAG probe, DiPP) for monitoring PNAG production and localization during biofilm formation. Mutation of the active site of Dispersin B gave DiPP, which was an effective probe despite its low affinity for PNAG oligosaccharides (K D ∼ 1–10 mM). Imaging of PNAG-dependent and -independent biofilms stained with a fluorescent-protein fusion of DiPP (GFP-DiPP) demonstrated the specificity of the probe for the structure of PNAG on both single-cell and biofilm levels, indicating a high local concentration of PNAG at the bacterial cell surface. Through quantitative bacterial cell binding assays and confocal microscopy analysis using GFP-DiPP, discrete areas of local high concentrations of PNAG were detected on the surface of early log phase cells. These distinct areas were seen to grow, slough from cells, and accumulate in interbacterial regions over the course of several cell divisions, showing the development of a PNAG-dependent biofilm. A potential helical distribution of staining was also noted, suggesting some degree of organization of PNAG production at the cell surface prior to cell aggregation. Together, these experiments shed light on the early stages of PNAG-dependent biofilm formation and demonstrate the value of a low-affinity-high-specificity probe for monitoring the production of bacterial exopolysaccharides