Listeria monocytogenes in sliced cheese and ham from retail markets in southern Brazil

ABSTRACT The aims of this study were to evaluate the occurrence of Listeria monocytogenes and Salmonella spp. in sliced cheese and ham from retail markets in southern Brazil, as well as to perform molecular characterization and to assess the antimicrobial resistance profile of the isolates. Samples...

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Bibliographic Details
Published in:FEMS microbiology letters 2019-11, Vol.366 (22)
Main Authors: Maia, Darla Silveira Volcan, Haubert, Louise, Würfel, Simone de Fátima Rauber, Kroning, Isabela Schneid, Cardoso, Marisa Ribeiro de Itapema, Lopes, Graciela Völz, Fiorentini, Ângela Maria, da Silva, Wladimir Padilha
Format: Article
Language:English
Subjects:
Adaptability
Antimicrobial agents
Antimicrobial resistance
Asci
Cheese
Clindamycin
Dairy products
Electrophoresis
Food irradiation
Gel electrophoresis
Genes
Listeria
Listeria monocytogenes
Microbiology
Public health
Pulsed-field gel electrophoresis
Salmonella
Streptomycin
Virulence
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Summary:ABSTRACT The aims of this study were to evaluate the occurrence of Listeria monocytogenes and Salmonella spp. in sliced cheese and ham from retail markets in southern Brazil, as well as to perform molecular characterization and to assess the antimicrobial resistance profile of the isolates. Samples (n = 160) of sliced cheese and ham were collected at retail level from the city of Pelotas, Brazil. The isolation of L. monocytogenes and Salmonella spp. was performed and the isolates were confirmed by PCR, submitted to antimicrobial susceptibility testing and pulsed-field gel electrophoresis (PFGE). Listeria monocytogenes was found in 9.4% (15/160) of the samples. All L. monocytogenes isolates were positive for the prs, inlA, inlC and inlJ genes. Salmonella spp. was not isolated. Regarding the antimicrobial susceptibility, one (6.6%) L. monocytogenes isolate was resistant to streptomycin and four (26.6%) to clindamycin. Macrorestriction analysis with ApaI and AscI enzymes yielded two major PFGE groups I and II. All L. monocytogenes isolates showed virulence genes, and some of them were resistant to clinically used antimicrobials, representing a risk to public health. Moreover, PFGE patterns with high similarity were visualized in L. monocytogenes isolates at different times, demonstrating adaptability of the pathogen at retail level in the region. Listeria monocytogenes in ready-to-eat foods
ISSN:1574-6968
0378-1097
1574-6968
DOI:10.1093/femsle/fnz249