Multiparametric Analysis of Tissue Spheroids Fabricated from Different Types of Cells

Reproducible, scalable, and cost effective fabrication and versatile characterization of tissue spheroids (TS) is highly demanded by 3D bioprinting and drug discovery. Consistent geometry, defined mechanical properties, optimal viability, appropriate extracellular matrix/cell organization are requir...

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Bibliographic Details
Published in:Biotechnology journal 2020-05, Vol.15 (5), p.e1900217-n/a
Main Authors: Koudan, Elizaveta V., Gryadunova, Anna A., Karalkin, Pavel A., Korneva, Janetta V., Meteleva, Nina Y., Babichenko, Igor I., Volkov, Aleksey V., Rodionov, Sergey A., Parfenov, Vladislav A., Pereira, Frederico D. A. S., Khesuani, Yusef D., Mironov, Vladimir A., Bulanova, Elena A.
Format: Article
Language:English
Subjects:
Animals
biofabrication
Biomarkers - metabolism
biomechanics
Bioprinting
Cell Line
Cell Survival
Fibroblasts - cytology
Fibroblasts - metabolism
HEK293 Cells
high‐scale production
Humans
Rats
Spheroids, Cellular - cytology
Spheroids, Cellular - metabolism
Tissue Engineering - methods
tissue spheroids
viability
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Summary:Reproducible, scalable, and cost effective fabrication and versatile characterization of tissue spheroids (TS) is highly demanded by 3D bioprinting and drug discovery. Consistent geometry, defined mechanical properties, optimal viability, appropriate extracellular matrix/cell organization are required for cell aggregates aimed for application in these fields. A straightforward procedure for fabrication and systematic multiparametric characterization of TS with defined properties and uniform predictable geometry employing non‐adhesive technology is suggested. Applying immortalized and primary cells, the reproducibility of spheroid generation, the strong correlation of ultimate spheroid diameter, and growth pattern with cell type and initial seeding concentration are demonstrated. Spheroids viability and mechanical properties are governed by cell derivation. In this study, a new decision procedure to apply for any cell type one starts to work with to prepare and typify TS meeting high quality standards in biofabrication and drug discovery is suggested. Tissue spheroids (TS) is a powerful tool for creation of human tissues. In this study, authors suggest a procedure for multiparametric characterization of TS to generate cell aggregates with defined properties and uniform predictable geometry employing non‐adhesive technology. This knowledge will allow to fabricate viable TS with optimal diameter and appropriate biomechanics for the application in biofabrication and drug discovery.
ISSN:1860-6768
1860-7314
DOI:10.1002/biot.201900217