Development and full validation of a multiresidue method for the analysis of a wide range of pesticides in processed fruit by UHPLC-MS/MS

• Published in: Food chemistry 2020-06, Vol.315, p.126304-126304, Article 126304
• Main Authors: Valera-Tarifa, N.M., Santiago-Valverde, R., Hernández-Torres, E., Martínez-Vidal, J.L., Garrido-Frenich, A.
• Format: Article
• Language: English
• Subjects: Mass spectrometry; Pesticides; Processed fruit; QuEChERS; Routine analysis; Ultra-performance liquid chromatography
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2020.126304

•First UHPLC-MS/MS method for analysis >200 pesticides in complex processed fruit.•Method fully validated by SANTE 11813/2017 document and accredited by ISO/IEC 17025.•Pure, concentrated pure, jam, pulp and fruit in syrup were analyzed.•Spinosad and fluopyram were the most detected but below the MRLs (raw fruit) A reliable 16-min analytical method for the simultaneous determination of 250 pesticides in processed fruit using ultra-performance liquid chromatography, coupled to tandem mass spectrometry (UHPLC–MS/MS), was developed and validated according to SANTE 11813/2017 guidelines and accredited successfully based on ISO 17025. Extraction was achieved using a modified QuEChERS method, without any clean-up, including a dilution to obtain good peak shapes and to reduce matrix effects. Pesticides were quantified using matrix-matched calibration, and the method was validated in terms of relative retention time window, linearity (6–167 µg kg−1 and 0.6–16.7 µg kg−1, coefficient R2 ≥ 0.98), trueness (recovery of 70–120%), selectivity, precision (RSD ≤ 20%), limits of quantification (LOQs = 0.6–6.0 µg kg−1) and uncertainty. Finally, the method was applied to the routine analysis of 103 samples of processed fruits, detecting the presence of several pesticide residues, such as fluopyram, spinosad or cyprodinil (0.006–0.22 mg kg−1).