Optimization of the Ames RAMOS test allows for a reproducible high-throughput mutagenicity test

• Published in: The Science of the total environment 2020-05, Vol.717, p.137168-137168, Article 137168
• Main Authors: Kauffmann, Kira, Werner, Felix, Deitert, Alexander, Finklenburg, Julian, Brendt, Julia, Schiwy, Andreas, Hollert, Henner, Büchs, Jochen
• Format: Article
• Language: English
• Subjects: Ames test; Histidine; Mutagenicity; Mutagenicity Tests; Mutagens; Oxygen transfer rate; RAMOS; Reproducibility of Results; Salmonella typhimurium
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2020.137168

The Ames test is one of the most widely used mutagenicity tests. It employs histidine auxotrophic bacteria, which can mutate back to histidine prototrophy and, thus, grow on a histidine deficient medium. These mutants develop predominantly after adding a mutagenic compound during an initial growth phase on 1 mg/L histidine. In the established test systems, an endpoint determination is performed to determine the relative number of mutants. An alternative Ames test, the Ames RAMOS test, has been developed, which enables the online detection of mutagenicity by monitoring respiration activity. The reproducibility of the newly developed test system was investigated. A strong dependence of the test results on the inoculum volume transferred from the preculture was found. The more inoculum was needed to reach the required initial OD, the more mutagenic a positive control was evaluated. This effect was attributed to the histidine transfer from the preculture to the original Ames RAMOS test. The same problem is evident in the Ames fluctuation test. High reproducibility of the Ames RAMOS test could be achieved by performing the preculture on minimal medium with a defined histidine concentration and termination after histidine depletion. By using 5 mg/L initial histidine within the minimal medium, a higher separation efficiency between negative control and mutagenic samples could be achieved. This separation efficiency could be further increased by lowering the cultivation temperature from 37 to 30 °C, i.e. lowering the maximum growth rate. The optimized Ames RAMOS test was then transferred into a 48-well microtiter plate format (μRAMOS) for obtaining a high throughput test. The online detection of mutagenicity leads to a reduction of working time in the laboratory. Due to the optimization of reproducibility and the increase in separation efficiency, a sound mutagenicity evaluation, even of weak mutagenic compounds, can be achieved. [Display omitted] •Newly developed Ames RAMOS test allows online detection of mutagenicity.•Observed variability due to histidine transfer from preculture could be eliminated.•A higher initial histidine concentration increases sensitivity of Ames RAMOS test.•A lower cultivation temperature increases sensitivity of Ames RAMOS test.•Successful generation of dose response data in high throughput Ames RAMOS test