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The increase in bone resorption in early-stage type I diabetic mice is induced by RANKL secreted by increased bone marrow adipocytes

Bone marrow adipose tissue (BMAT) has recently been found to induce osteoclastogenesis by secreting RANKL. Although Type 1 diabetes mellitus (T1DM) has been reported to be associated with increased BMAT and bone loss, little is known about the relationship between BMAT and osteoclasts in T1DM. We st...

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Published in:Biochemical and biophysical research communications 2020-04, Vol.525 (2), p.433-439
Main Authors: Yang, Jiazhi, Chen, Sixu, Zong, Zhaowen, Yang, Lei, Liu, Daocheng, Bao, Quanwei, Du, Wenqiong
Format: Article
Language:English
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Summary:Bone marrow adipose tissue (BMAT) has recently been found to induce osteoclastogenesis by secreting RANKL. Although Type 1 diabetes mellitus (T1DM) has been reported to be associated with increased BMAT and bone loss, little is known about the relationship between BMAT and osteoclasts in T1DM. We studied the role of BMAT in the alterations of osteoclast activities in early-stage T1DM, by using a streptozotocin-induced T1DM mouse model. Our results showed that osteoclast activity was enhanced in the long bones of T1DM mice, accompanied by increased protein expression of RANKL. However, RANKL mRNA levels in bone tissues of T1DM mice remained unchanged. Meanwhile, we found that BMAT was significantly increased in the long bones of T1DM mice, and both mRNA and protein levels of RANKL were elevated in the diabetic BMAT. More importantly, RANKL protein was mainly expressed on the cell membranes of the increased adipocytes, most of which were located next to the metaphyseal region. These results suggest that the enhanced bone resorption in early-stage diabetic mice is induced by RANKL derived from BMAT rather than the bone tissue itself. •The osteoclast activity was enhanced in the long bones of T1DM mice, while the RANKL expression levels remained unchanged in bone tissues.•The enhanced bone resorption in early-stage diabetic mice is induced by RANKL derived from BMAT rather than the bone tissue itself.•BMAT has an underestimated potential to regulate bone homeostasis in diabetic osteopenia, which may be useful for developing novel treatments.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2020.02.079