Tissue-specific transcriptional regulation and metabolite accumulation in tomato (Solanum lycopersicum L.)

• Published in: Protoplasma 2020-07, Vol.257 (4), p.1093-1108
• Main Authors: Sharma, Deepak, Koul, Archana, Kaul, Sanjana, Dhar, Manoj K.
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Cell Biology; DNA microarrays; Flavonoids; Fruit - chemistry; Fruits; Gene expression; Gene Expression Regulation, Plant - genetics; Gene regulation; Genetic control; Life Sciences; Lycopersicon esculentum - chemistry; Metabolic pathways; Metabolism; Metabolites; Molecular modelling; Oligonucleotides; Original Article; Pigments; Plant Sciences; Ripening; Solanum lycopersicum; Transcription factors; Transcription Factors - metabolism; Zoology
• ISSN: 0033-183X; 1615-6102
• DOI: 10.1007/s00709-020-01492-2

Tomato is an excellent model for studying fruit development, ripening, and other secondary metabolic pathways such as carotenoid biosynthetic pathway, flavonoid pathway, and many more. Tomato fruit development and ripening occurs under tight genetic control and involves the expression of thousands of genes affecting fruit quality and accumulation of pigments and metabolites. Here, we have described the development of a microarray platform that has allowed establishment of a framework for quantification of the expression of large number of genes and transcription factors possibly regulating various secondary metabolic pathways in tomato. To unravel the molecular mechanisms of fruit development and ripening, a tomato 60-mer oligonucleotide 44 K microarray along with the custom array for many genes and transcription factors was designed and validated in the fruit and leaf tissues. Comparative profiling of gene expression studies has allowed us to identify a large number of differentially expressed genes and transcription factors. Gene ontology revealed the involvement of these genes in various biological, cellular, and molecular processes like isoprenoid, terpenoid, pigment, ethylene biosynthesis, phytohormone signaling, and fruit ripening. Further, correlation, as well as differential expression studies, has revealed that several transcription factors like RIN, AGAMOUS, TAGL1, MYB, MADS-box etc. could be the possible regulators of various secondary metabolic pathways. The present study has identified various metabolites, their biosynthetic pathways and genes which may possibly be controlled by different transcription factors. The present findings have laid a base for understanding the transcriptional and metabolic shifts which occur in parallel during programmed fruit ripening and developmental processes in tomato.