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Virulence Genes Analysis of Vibrio parahaemolyticus and Anti-vibrio Activity of the Citrus Extracts
Vibrio parahaemolyticus (VP) is a marine bacterium that opportunistically caused foodborne gastroenteritis in human and some diseases in marine animals. The isolated strain of V. parahaemolyticus WS001 from Samut Sakhon, Thailand has a presence of ldh (~ 450 bp) tox A (~ 333 bp) and tox B (~ 1269 bp...
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| Published in: | Current microbiology 2020-08, Vol.77 (8), p.1390-1398 |
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| container_title | Current microbiology |
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| creator | Singhapol, Chatchawan Tinrat, Sirikhwan |
| description | Vibrio parahaemolyticus
(VP) is a marine bacterium that opportunistically caused foodborne gastroenteritis in human and some diseases in marine animals. The isolated strain of
V. parahaemolyticus
WS001 from Samut Sakhon, Thailand has a presence of
ldh
(~ 450 bp)
tox
A (~ 333 bp) and
tox
B (~ 1269 bp) genes which showed pathogenicity in shrimp. This strain is suspected as low pathogenicity in human due to the lack of
tdh
and
trh
genes for encoding thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH), respectively. The shrimp pathogenic strain was tested and revealed the multi-antibiotic resistances but was susceptible to norfloxacin (10 μg/ml).
Citrus
peel extracts were examined because they are rich in bioactive compounds such as saponins, tannins, flavonoids, steroids, and alkaloids that are effective in anti-VP activities. The ethanolic peel extracts of
Citrus aurantifolia
(Christm.) Swingle and
Citrus hystrix
DC. were found to be more anti-VP effect than other solvent extracts by Agar disc diffusion method at an optimum concentration of 50 mg/ml and Broth micro-dilution method (MICs of 50–100 mg/ml and MBCs of 100–200 mg/ml). Thus,
C. aurantifolia
(Christm.) Swingle peel extract was a distinctive candidate for the development of alternative natural agent to control the spreading of diseases in shrimp. |
| doi_str_mv | 10.1007/s00284-020-01941-4 |
| format | article |
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(VP) is a marine bacterium that opportunistically caused foodborne gastroenteritis in human and some diseases in marine animals. The isolated strain of
V. parahaemolyticus
WS001 from Samut Sakhon, Thailand has a presence of
ldh
(~ 450 bp)
tox
A (~ 333 bp) and
tox
B (~ 1269 bp) genes which showed pathogenicity in shrimp. This strain is suspected as low pathogenicity in human due to the lack of
tdh
and
trh
genes for encoding thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH), respectively. The shrimp pathogenic strain was tested and revealed the multi-antibiotic resistances but was susceptible to norfloxacin (10 μg/ml).
Citrus
peel extracts were examined because they are rich in bioactive compounds such as saponins, tannins, flavonoids, steroids, and alkaloids that are effective in anti-VP activities. The ethanolic peel extracts of
Citrus aurantifolia
(Christm.) Swingle and
Citrus hystrix
DC. were found to be more anti-VP effect than other solvent extracts by Agar disc diffusion method at an optimum concentration of 50 mg/ml and Broth micro-dilution method (MICs of 50–100 mg/ml and MBCs of 100–200 mg/ml). Thus,
C. aurantifolia
(Christm.) Swingle peel extract was a distinctive candidate for the development of alternative natural agent to control the spreading of diseases in shrimp.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s00284-020-01941-4</identifier><identifier>PMID: 32179973</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Alkaloids ; Animal diseases ; Animals ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Bacteria ; Bioactive compounds ; Biomedical and Life Sciences ; Biotechnology ; Citrus - chemistry ; Citrus aurantifolia ; Citrus hystrix ; Dilution ; Disease ; Drug Resistance, Multiple, Bacterial ; Epidemics ; Flavonoids ; Fruits ; Gastroenteritis ; Genes ; Life Sciences ; Marine animals ; Marine organisms ; Microbial Sensitivity Tests ; Microbiology ; Mortality ; Norfloxacin ; Pathogenicity ; Pathogens ; Penaeidae - microbiology ; Phytochemicals ; Plant extracts ; Plant Extracts - pharmacology ; Saponins ; Shellfish ; Steroid hormones ; Steroids ; Tannins ; Thailand ; Vibrio parahaemolyticus ; Vibrio parahaemolyticus - drug effects ; Vibrio parahaemolyticus - genetics ; Vibrio parahaemolyticus - pathogenicity ; Virulence ; Virulence Factors - genetics ; Waterborne diseases</subject><ispartof>Current microbiology, 2020-08, Vol.77 (8), p.1390-1398</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-713ee76ad6cdfa1250a9aad7a32e09550f2986c0888b37830021ad7bab9807f53</citedby><cites>FETCH-LOGICAL-c375t-713ee76ad6cdfa1250a9aad7a32e09550f2986c0888b37830021ad7bab9807f53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32179973$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Singhapol, Chatchawan</creatorcontrib><creatorcontrib>Tinrat, Sirikhwan</creatorcontrib><title>Virulence Genes Analysis of Vibrio parahaemolyticus and Anti-vibrio Activity of the Citrus Extracts</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><addtitle>Curr Microbiol</addtitle><description>Vibrio parahaemolyticus
(VP) is a marine bacterium that opportunistically caused foodborne gastroenteritis in human and some diseases in marine animals. The isolated strain of
V. parahaemolyticus
WS001 from Samut Sakhon, Thailand has a presence of
ldh
(~ 450 bp)
tox
A (~ 333 bp) and
tox
B (~ 1269 bp) genes which showed pathogenicity in shrimp. This strain is suspected as low pathogenicity in human due to the lack of
tdh
and
trh
genes for encoding thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH), respectively. The shrimp pathogenic strain was tested and revealed the multi-antibiotic resistances but was susceptible to norfloxacin (10 μg/ml).
Citrus
peel extracts were examined because they are rich in bioactive compounds such as saponins, tannins, flavonoids, steroids, and alkaloids that are effective in anti-VP activities. The ethanolic peel extracts of
Citrus aurantifolia
(Christm.) Swingle and
Citrus hystrix
DC. were found to be more anti-VP effect than other solvent extracts by Agar disc diffusion method at an optimum concentration of 50 mg/ml and Broth micro-dilution method (MICs of 50–100 mg/ml and MBCs of 100–200 mg/ml). Thus,
C. aurantifolia
(Christm.) Swingle peel extract was a distinctive candidate for the development of alternative natural agent to control the spreading of diseases in shrimp.</description><subject>Alkaloids</subject><subject>Animal diseases</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Bioactive compounds</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Citrus - chemistry</subject><subject>Citrus aurantifolia</subject><subject>Citrus hystrix</subject><subject>Dilution</subject><subject>Disease</subject><subject>Drug Resistance, Multiple, Bacterial</subject><subject>Epidemics</subject><subject>Flavonoids</subject><subject>Fruits</subject><subject>Gastroenteritis</subject><subject>Genes</subject><subject>Life Sciences</subject><subject>Marine animals</subject><subject>Marine organisms</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Mortality</subject><subject>Norfloxacin</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Penaeidae - microbiology</subject><subject>Phytochemicals</subject><subject>Plant extracts</subject><subject>Plant Extracts - pharmacology</subject><subject>Saponins</subject><subject>Shellfish</subject><subject>Steroid hormones</subject><subject>Steroids</subject><subject>Tannins</subject><subject>Thailand</subject><subject>Vibrio parahaemolyticus</subject><subject>Vibrio parahaemolyticus - drug effects</subject><subject>Vibrio parahaemolyticus - genetics</subject><subject>Vibrio parahaemolyticus - pathogenicity</subject><subject>Virulence</subject><subject>Virulence Factors - genetics</subject><subject>Waterborne diseases</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kb1OwzAURi0EglJ4AQYUiYXFcG0nsT1WVSlISCzQ1XIcB4zSpNgOom-PIfxIDEwe7jmfde-H0AmBCwLALwMAFTkGChiIzAnOd9CE5IxikJLsogmwnGFRFuQAHYbwDECoBLKPDhglXErOJsisnB9a2xmbLW1nQzbrdLsNLmR9k61c5V2fbbTXT9qu-3YbnRlCprs6cdHh1xGYmeheXdx-OPHJZnMXfcIWb9FrE8MR2mt0G-zx1ztFD1eL-_k1vr1b3sxnt9gwXkTMCbOWl7ouTd1oQgvQUuuaa0YtyKKAhkpRGhBCVIwLlrYnaVzpSgrgTcGm6HzM3fj-ZbAhqrULxrat7mw_BEWTBQBlcqfo7A_63A8-rZ6onEgu0oHLRNGRMr4PwdtGbbxba79VBNRHBWqsQKUK1GcFKk_S6Vf0UK1t_aN83zwBbARCGnWP1v_-_U_sO0pekPE</recordid><startdate>20200801</startdate><enddate>20200801</enddate><creator>Singhapol, Chatchawan</creator><creator>Tinrat, Sirikhwan</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20200801</creationdate><title>Virulence Genes Analysis of Vibrio parahaemolyticus and Anti-vibrio Activity of the Citrus Extracts</title><author>Singhapol, Chatchawan ; Tinrat, Sirikhwan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-713ee76ad6cdfa1250a9aad7a32e09550f2986c0888b37830021ad7bab9807f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Alkaloids</topic><topic>Animal diseases</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - 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drug effects</topic><topic>Vibrio parahaemolyticus - genetics</topic><topic>Vibrio parahaemolyticus - pathogenicity</topic><topic>Virulence</topic><topic>Virulence Factors - genetics</topic><topic>Waterborne diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Singhapol, Chatchawan</creatorcontrib><creatorcontrib>Tinrat, Sirikhwan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest research library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Singhapol, Chatchawan</au><au>Tinrat, Sirikhwan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Virulence Genes Analysis of Vibrio parahaemolyticus and Anti-vibrio Activity of the Citrus Extracts</atitle><jtitle>Current microbiology</jtitle><stitle>Curr Microbiol</stitle><addtitle>Curr Microbiol</addtitle><date>2020-08-01</date><risdate>2020</risdate><volume>77</volume><issue>8</issue><spage>1390</spage><epage>1398</epage><pages>1390-1398</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><abstract>Vibrio parahaemolyticus
(VP) is a marine bacterium that opportunistically caused foodborne gastroenteritis in human and some diseases in marine animals. The isolated strain of
V. parahaemolyticus
WS001 from Samut Sakhon, Thailand has a presence of
ldh
(~ 450 bp)
tox
A (~ 333 bp) and
tox
B (~ 1269 bp) genes which showed pathogenicity in shrimp. This strain is suspected as low pathogenicity in human due to the lack of
tdh
and
trh
genes for encoding thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH), respectively. The shrimp pathogenic strain was tested and revealed the multi-antibiotic resistances but was susceptible to norfloxacin (10 μg/ml).
Citrus
peel extracts were examined because they are rich in bioactive compounds such as saponins, tannins, flavonoids, steroids, and alkaloids that are effective in anti-VP activities. The ethanolic peel extracts of
Citrus aurantifolia
(Christm.) Swingle and
Citrus hystrix
DC. were found to be more anti-VP effect than other solvent extracts by Agar disc diffusion method at an optimum concentration of 50 mg/ml and Broth micro-dilution method (MICs of 50–100 mg/ml and MBCs of 100–200 mg/ml). Thus,
C. aurantifolia
(Christm.) Swingle peel extract was a distinctive candidate for the development of alternative natural agent to control the spreading of diseases in shrimp.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>32179973</pmid><doi>10.1007/s00284-020-01941-4</doi><tpages>9</tpages></addata></record> |
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| issn | 0343-8651 1432-0991 |
| language | eng |
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| subjects | Alkaloids Animal diseases Animals Anti-Bacterial Agents - pharmacology Antibiotics Bacteria Bioactive compounds Biomedical and Life Sciences Biotechnology Citrus - chemistry Citrus aurantifolia Citrus hystrix Dilution Disease Drug Resistance, Multiple, Bacterial Epidemics Flavonoids Fruits Gastroenteritis Genes Life Sciences Marine animals Marine organisms Microbial Sensitivity Tests Microbiology Mortality Norfloxacin Pathogenicity Pathogens Penaeidae - microbiology Phytochemicals Plant extracts Plant Extracts - pharmacology Saponins Shellfish Steroid hormones Steroids Tannins Thailand Vibrio parahaemolyticus Vibrio parahaemolyticus - drug effects Vibrio parahaemolyticus - genetics Vibrio parahaemolyticus - pathogenicity Virulence Virulence Factors - genetics Waterborne diseases |
| title | Virulence Genes Analysis of Vibrio parahaemolyticus and Anti-vibrio Activity of the Citrus Extracts |
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