Stimulation of water and calcium dynamics in astrocytes with pulsed infrared light

Astrocytes are non‐neuronal cells that govern the homeostatic regulation of the brain through ions and water transport, and Ca2+‐mediated signaling. As they are tightly integrated into neural networks, label‐free tools that can modulate cell function are needed to evaluate the role of astrocytes in...

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Bibliographic Details
Published in:The FASEB journal 2020-05, Vol.34 (5), p.6539-6553
Main Authors: Borrachero‐Conejo, Ana I., Adams, Wilson R., Saracino, Emanuela, Mola, Maria Grazia, Wang, Manqing, Posati, Tamara, Formaggio, Francesco, De Bellis, Manuela, Frigeri, Antonio, Caprini, Marco, Hutchinson, Mark R., Muccini, Michele, Zamboni, Roberto, Nicchia, Grazia Paola, Mahadevan‐Jansen, Anita, Benfenati, Valentina
Format: Article
Language:English
Subjects:
Animals
AQP4
Aquaporin 4 - genetics
Aquaporin 4 - metabolism
astrocytes
Astrocytes - cytology
Astrocytes - metabolism
Astrocytes - radiation effects
Biological Transport
Calcium - metabolism
Cells, Cultured
Homeostasis
Infrared Rays
infrared stimulation
Rats
Signal Transduction
TRPA1 Cation Channel - genetics
TRPA1 Cation Channel - metabolism
TRPV Cation Channels - genetics
TRPV Cation Channels - metabolism
TRPV4
Water - metabolism
water dynamics
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Summary:Astrocytes are non‐neuronal cells that govern the homeostatic regulation of the brain through ions and water transport, and Ca2+‐mediated signaling. As they are tightly integrated into neural networks, label‐free tools that can modulate cell function are needed to evaluate the role of astrocytes in brain physiology and dysfunction. Using live‐cell fluorescence imaging, pharmacology, electrophysiology, and genetic manipulation, we show that pulsed infrared light can modulate astrocyte function through changes in intracellular Ca2+ and water dynamics, providing unique mechanistic insight into the effect of pulsed infrared laser light on astroglial cells. Water transport is activated and, IP3R, TRPA1, TRPV4, and Aquaporin‐4 are all involved in shaping the dynamics of infrared pulse‐evoked intracellular calcium signal. These results demonstrate that astrocyte function can be modulated with infrared light. We expect that targeted control over calcium dynamics and water transport will help to study the crucial role of astrocytes in edema, ischemia, glioma progression, stroke, and epilepsy.
ISSN:0892-6638
1530-6860
1530-6860
DOI:10.1096/fj.201903049R