αB-crystallin response to a pro-oxidant non-cytotoxic environment in murine cardiac cells: An “in vitro” and “in vivo” study

The αB-crystallin (HSPB5) protein is modulated in response to a wide variety of stressors generated by multiple physio-pathological conditions, sustained by reactive oxygen species (ROS) production. In cardiac muscle tissue, this protein regulates various cellular processes, such as protein degradat...

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Bibliographic Details
Published in:Free radical biology & medicine 2020-05, Vol.152, p.301-312
Main Authors: Antonioni, Ambra, Dimauro, Ivan, Fantini, Cristina, Barone, Rosario, Macaluso, Filippo, Di Felice, Valentina, Caporossi, Daniela
Format: Article
Language:English
Subjects:
Cardiac muscle
Endurance exercise
HSPB5
Oxidative eustress
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Summary:The αB-crystallin (HSPB5) protein is modulated in response to a wide variety of stressors generated by multiple physio-pathological conditions, sustained by reactive oxygen species (ROS) production. In cardiac muscle tissue, this protein regulates various cellular processes, such as protein degradation, apoptosis and the stabilization of cytoskeletal elements. In this work, we studied the role of HSPB5 expression, activation and localization in HL-1 murine cardiomyocytes exposed to pro-oxidant and non-cytotoxic H2O2 concentration, as well as in cardiac tissue isolated from mice following an acute, non-damaging endurance exercise. Our results demonstrated that HSPB5 is the most abundant HSP in both cardiac muscle tissue and HL-1 cells when compared to HSPB1 or HSPA1A (≈3–8 fold higher protein concentrations, p 
ISSN:0891-5849
1873-4596
DOI:10.1016/j.freeradbiomed.2020.03.013