Seasonal shifts of arbuscular mycorrhizal fungi in Cocos nucifera roots in Yucatan, Mexico

• Published in: Mycorrhiza 2020-05, Vol.30 (2-3), p.269-283
• Main Authors: Lara-Pérez, Luis A., Oros-Ortega, Iván, Córdova-Lara, Iván, Estrada-Medina, Héctor, O’Connor-Sánchez, Aileen, Góngora-Castillo, Elsa, Sáenz-Carbonell, Luis
• Format: Article
• Language: English
• Subjects: Agriculture; Arbuscular mycorrhizas; Biodiversity; Biomedical and Life Sciences; Cocos; Cocos nucifera; Community structure; Composition; Dry forests; Dry season; Dunes; Ecology; Forestry; Fungi; Glomeromycota; Life Sciences; Mexico; Microbiology; Morphology; Mycorrhizae; New genera; Next-generation sequencing; Original Article; Plant Roots; Plant Sciences; Plantations; Rainy season; Rhizosphere; Roots; Sclerocystis; Seasons; Soil Microbiology; Soils; Sustainable agriculture; Tropical forests
• ISSN: 0940-6360; 1432-1890
• DOI: 10.1007/s00572-020-00944-0

The diversity and community structure of arbuscular mycorrhizal fungi (AMF) associated with coconut ( Cocos nucifera ) roots was evaluated by next generation sequencing (NGS) using partial sequences of the 18S rDNA gene and by spore isolation and morphological identification from rhizosphere soil. Root samples from six different Green Dwarf coconut plantations and from one organic plantation surrounded by tropical dry forest along the coastal sand dunes in Yucatan, Mexico, were collected during the rainy and dry seasons. In total, 14 root samples were sequenced with the Illumina MiSeq platform. Additionally, soil samples from the dry season were collected to identify AMF glomerospores. Based on a 95–97% similarity, a total of 36 virtual taxa (VT) belonging to nine genera were identified including one new genus-like clade. Glomus was the most abundant genus, both in number of VT and sequences. The comparison of dry and rainy season samples revealed differences in the richness and composition of AMF communities colonizing coconut roots. Our study shows that the main AMF genera associated with coconut tree roots in all samples were Glomus , Sclerocystis , Rhizophagus , Redeckera , and Diversispora. Based on glomerospore morphology, 22 morphospecies were recorded among which 14 were identified to species. Sclerocystis sinuosa , Sclerocystis rubiformis , Glomus microaggregatum , and Acaulospora scrobiculata were dominant in field rhizosphere samples. This is the first assessment of the composition of AMF communities colonizing coconut roots in rainy and dry seasons. It is of importance for selection of AMF species to investigate for their potential application in sustainable agriculture of coconut.