A microfluidic chip with double-sided herringbone microstructures for enhanced capture of rare tumor cells

A microfluidic chip with single-sided herringbone microstructure has been developed to isolate circulating tumor cells (CTCs) from blood samples of cancer patients. Here, we describe a new double-sided herringbone chip in which staggered herringbone micromixers are placed on both top and bottom surf...

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Bibliographic Details
Published in:Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2017-12, Vol.5 (46), p.9114-912
Main Authors: Wang, Minjiao, Wang, Zhihua, Zhang, Mingkan, Guo, Wei, Li, Ning, Deng, Yuliang, Shi, Qihui
Format: Article
Language:English
Subjects:
Blood circulation
Cancer
Efficiency
Erythrocytes
Leukocytes
Mathematical models
Microchannels
Microfluidics
Optimization
Parameters
Tumor cells
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Summary:A microfluidic chip with single-sided herringbone microstructure has been developed to isolate circulating tumor cells (CTCs) from blood samples of cancer patients. Here, we describe a new double-sided herringbone chip in which staggered herringbone micromixers are placed on both top and bottom surfaces of microchannels. The double-sided herringbone structure enables a high CTC capture efficiency of whole blood samples without depletion of red blood cells because of the effects of leukocyte margination and plasma skimming. However, compared with the traditional single-sided herringbone chip, the double-sided herringbone chip has more complicated geometrical design, leading to a difficulty in experimental optimization of geometrical parameters. In this study, we developed an analytical model to geometrically optimize the herringbone chip by investigating the interactions between cells and antibody-immobilized device surfaces for enhancing CTC capture efficiency. On-chip cell capture experiments for validating modeling results were performed by spiking cultured EpCAM-positive tumor cells into blood samples from healthy donors. Based on the geometrical parameters optimized from the single-sided herringbone chip, the geometrically optimized double-sided herringbone chip enables a capture efficiency of 94 ± 4% of rare tumor cells directly from whole blood. This paper reports an analytical model to geometrically optimize a herringbone chip by investigating the interactions between cells and antibody-immobilized device surfaces for enhancing CTC capture efficiency.
ISSN:2050-750X
2050-7518
DOI:10.1039/c7tb02318a