Genomic characterisation of a newly identified badnavirus infecting ivy (Hedera helix)

High-throughput sequencing (HTS) was used to investigate ringspots on ivy ( Hedera helix ) leaves. De novo assembly of HTS data generated from a total RNA extract from these leaves yielded a contig with sequence similarity to viruses of the genus Badnavirus, family Caulimoviridae . The complete geno...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 2020-06, Vol.165 (6), p.1511-1514
Main Authors: Bester, Rachelle, Burger, Johan T., Maree, Hans J.
Format: Article
Language:English
Subjects:
Annotated Sequence Record
Badnavirus
Badnavirus - classification
Badnavirus - genetics
Badnavirus - isolation & purification
Biomedical and Life Sciences
Biomedicine
Genome, Viral
Genomes
Hedera - virology
Hedera helix
High-Throughput Nucleotide Sequencing
Infectious Diseases
Medical Microbiology
Next-generation sequencing
Nucleotide sequence
Open Reading Frames
Phylogeny
Plant Diseases - virology
Ribonucleic acid
RNA
Virology
Viruses
Whole Genome Sequencing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:High-throughput sequencing (HTS) was used to investigate ringspots on ivy ( Hedera helix ) leaves. De novo assembly of HTS data generated from a total RNA extract from these leaves yielded a contig with sequence similarity to viruses of the genus Badnavirus, family Caulimoviridae . The complete genome sequence of this virus consists of 8,885 nucleotides and has three open reading frames (ORFs). Genome organisation and phylogenetic analysis identifies this newly identified virus as a new member of the genus Badnavirus for which we propose the name "ivy ringspot-associated virus" (IRSaV).
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-020-04627-1