Direct Relationship Between Metabolic Flexibility Measured During Glucose Clamp and Prolonged Fast in Men

Objective This study aimed to determine the relationship between metabolic flexibility (MetFlex) measured during a euglycemic‐hyperinsulinemic clamp and a prolonged fast. This study also analyzed the association between MetFlex and metabolic health. Methods Eighteen healthy men (mean [SD]: 22 [2] ye...

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Published in:Obesity (Silver Spring, Md.) Md.), 2020-06, Vol.28 (6), p.1110-1116
Main Authors: Fernández‐Verdejo, Rodrigo, Castro‐Sepulveda, Mauricio, Gutiérrez‐Pino, Juan, Malo‐Vintimilla, Lorena, López‐Fuenzalida, Antonio, Olmos, Pablo, Santos, José L., Galgani, José E.
Format: Article
Language:English
Subjects:
Adult
Catheters
Cholesterol
Exercise
Fasting - physiology
Flexibility
Glucose
Glucose Clamp Technique - methods
Humans
Insulin - blood
Insulin resistance
Insulin Resistance - physiology
Lipids
Lipoproteins
Male
Metabolism
Physical fitness
Veins & arteries
Young Adult
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Summary:Objective This study aimed to determine the relationship between metabolic flexibility (MetFlex) measured during a euglycemic‐hyperinsulinemic clamp and a prolonged fast. This study also analyzed the association between MetFlex and metabolic health. Methods Eighteen healthy men (mean [SD]: 22 [2] years old; BMI: 22 [1] kg/m2) performed two sessions: (1) euglycemic‐hyperinsulinemic clamp (2 mIU/kg of insulin per minute) and (2) ~20‐hour fast. Clamp MetFlex corresponded to the change in (Δ) respiratory quotient (RQ) (ΔRQ = postchallenge RQ − prechallenge RQ) adjusted for M value and prechallenge RQ. Prolonged fast MetFlex corresponded to the ΔRQ adjusted for the Δβ‐hydroxybutyrate and prechallenge RQ. Results MetFlex during the clamp related directly with MetFlex during prolonged fast (r = 0.59, P = 0.014). Using the median of MetFlex for each challenge, this study split participants into high or low MetFlex. Participants with high or low MetFlex to both challenges were identified. Participants with high MetFlex had 3% lower serum low‐density lipoprotein cholesterol than participants with low MetFlex (P = 0.021). Conclusions Measuring MetFlex during a clamp or a prolonged fast produces similar results, despite challenging the oxidation of different substrates. An impaired MetFlex in response to these challenges may be an early event in the development of abnormal lipid metabolism.
ISSN:1930-7381
1930-739X
DOI:10.1002/oby.22783