Isolation and Identification of Plant Growth-Promoting Bacteria Highly Effective in Suppressing Root Rot in Fava Beans

• Published in: Current microbiology 2020-09, Vol.77 (9), p.2155-2165
• Main Authors: Saad, Mona M. G., Kandil, Mahrous, Mohammed, Youssef M. M.
• Format: Article
• Language: English
• Subjects: Acetic acid; Agrochemicals; Antifungal activity; Bacteria - genetics; Beans; Biomedical and Life Sciences; Biotechnology; Broad beans; Cotton; Damping; Exopolysaccharides; Fertilizers; Field tests; Fungicides; Indoleacetic acid; Life Sciences; Microbiology; Morphology; Pesticides; Phytopathogenic fungi; Plant Diseases; Plant growth; Plant Roots; Rhizoctonia; Rhizosphere; RNA, Ribosomal, 16S - genetics; Root rot; rRNA 16S; Silk; Strains (organisms); Vicia faba
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-020-02015-1

In this study, 18 plant growth-promoting bacterial (PGPB) strains were isolated from the rhizosphere of both Red silk-cotton tree ( Bombax malabaricum ) and Chinese banyan (Ficus retusa) . Culture morphology was observed and genotypic characterization was accomplished by sequencing partial 16S rRNA gene. Plant growth promotion traits and antagonistic activities of the strains against phytopathogenic fungi were evaluated. Among all strains, Bacillus thuringiensis (MN419208) exert the highest indole acetic acid (38 µg/ml), produced exopolysaccharides (587.2 µg/ml), and fixed nitrogen which in turn increased both fresh and dry weights of bean plants by 41.5% and 18.8%, respectively. In another greenhouse experiment studying the antifungal activities of seven strains and their co-culture against Rhizoctonia solani (LN735538), B. sonorensis MN419205, B. wiedmannii MN419207, B. subtilis MN419218, and the mixture of (MN419207) and (MN419208) reduced total damping off from 81.7% in control to 30%, 35%, 35%, and 38.3%, respectively, and reduced disease severity index from 33.3% to 20.5%, 22.5%, 14.2%, and 19.3% as well. Our data indicate that these strains are effective in promoting plant growth and in inhibiting R. solani infection nonetheless field experiments are needed to examine their effectiveness as a viable alternative to chemical fertilizers and conventional pesticides.