Porcine rotavirus C strains carrying human‐like NSP4 and NSP5

• Published in: Zoonoses and public health 2020-12, Vol.67 (8), p.849-861
• Main Authors: Costa, Fábio Burack, Flores, Patrícia Soares, Amorim, Ariane Ribeiro, Mendes, Gabriella da Silva, Santos, Norma
• Format: Article
• Language: English
• Subjects: Animals; Evolution; Feces - virology; Gene sequencing; Genetic diversity; Genetic variation; Genotype; Genotypes; Humans; interspecies transmission; NSP4 gene; Nucleotide sequence; Nucleotides; Pathogens; PCR; Phylogeny; Polymerase chain reaction; porcine; reassortment; Reverse transcription; Rotavirus; Rotavirus - genetics; Rotavirus Infections - veterinary; Rotavirus Infections - virology; RVC; Segments; Sequencing; Strains (organisms); Swine; Swine Diseases - virology; Toxins, Biological - genetics; Transcription; Viral Nonstructural Proteins - genetics; Viruses
• ISSN: 1863-1959; 1863-2378
• DOI: 10.1111/zph.12713

Background Rotavirus C (RVC) is an enteric pathogen that affects humans and animals around the world. Methods In this study, we characterized the genetic diversity of RVC strains detected in asymptomatic Brazilian pigs by sequencing the NSP4, NSP5 and VP6 genes. Results The results of reverse transcription polymerase chain reaction showed that 53 of 579 samples (9.2%) contained RVC. Positive samples were genotyped by sequencing gene segments NSP4, and NSP5. Most of the RCV strains encountered were classified into typically porcine genotypes: E1‐H1. In two strains, BP182 and BP208, the NSP4 gene grouped with E2‐RVC human strains with 94.2%–96.5% nucleotide identity, although the NSP5 gene was porcine‐like (H1). In strain SD67, the NSP5 gene grouped with human H2‐RVC with 92.5%–98.7% nucleotide identity and the NSP4 gene grouped with porcine strains (E1). Two strains (BP208 and SD67) were also genotyped by sequencing gene segment VP6. The VP6 gene grouped with porcine strains, I6 (89.3%–90.2% nucleotide identity) and I5 (88.7%–90.5% nucleotide identity), for strains BP208 and SD67, respectively. Conclusions These results are indicative of genomic reassortment between RVC strains of human and porcine origin. In recent years, the incidence of RVC infection among humans has increased significantly. It is important to measure the frequency of interspecies transmission in order to monitor the evolution of these viruses and to identify rearranged strains that may lead to an epidemic.