Preservation of Mesenchymal Stem Cell-Derived Extracellular Vesicles after Abdominal Delivery in the Experiment

• Published in: Bulletin of experimental biology and medicine 2020-05, Vol.169 (1), p.122-129
• Main Authors: Sukhikh, G. T., Pekarev, О. G., Maiborodin, I. V., Silachev, D. N., Shevtsova, Yu. А., Gоrуunоv, K. V., Onoprienko, N. V., Maiborodina, V. I., Galenok, R. V., Novikov, A. V., Pekareva, Е. О.
• Format: Article
• Language: English
• Subjects: Analysis; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cesarean section; Extracellular vesicles; Internal Medicine; Laboratory Medicine; Leukocyte migration; Light microscopy; Macrophages; Mesenchymal stem cells; Microscopy; Pathology; Phagocytosis; Placenta; Preservation; Stem cells; Stromal cells; Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine); Uterus
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-020-04838-1

Light luminescent microscopy was used to study the distribution of extracellular microvesicles with PKH26-stained membranes secreted by placenta-derived mesenchymal stromal cells in the uterine tissues at different terms after injections to intact rats and after abdominal delivery (a model of cesarian section). Microvesicles migrated through the uterine tissues and were detected for at least 8 days after injection. In some cases, microvesicles were more numerous in the uterus after cesarian section modeling, which can be related to blockade of microcirculation and lymph flow due to inflammation accompanying surgical intervention. The content of microvesicles in the uterine tissues gradually declined due to macrophage phagocytosis and, probably, due to their migration into the vascular bed. Despite their size, properly stained extracellular microvesicles can be detected by light microscopy in tissues after injections.