Bacterial compatibility and immobilization with biochar improved tebuconazole degradation, soil microbiome composition and functioning

• Published in: Journal of hazardous materials 2020-11, Vol.398, p.122941-122941, Article 122941
• Main Authors: Sun, Tong, Miao, Jingbo, Saleem, Muhammad, Zhang, Haonan, Yang, Yong, Zhang, Qingming
• Format: Article
• Language: English
• Subjects: Alcaligenes faecalisWZ-2; Bacteria - genetics; Bioremediation; Charcoal; Immobilization; Microbiota; Soil; Soil Microbiology; Soil microbiome; Soil Pollutants; Tebuconazole; Triazoles
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2020.122941

•A high-efficiency tebuconazole-degrading bacteria WZ-2 was isolated and identified.•The compatibility of strain WZ-2 with biochar was tested.•Tebuconazole decreased soil microbial activity and community.•Biochar-immobilized strain WZ-2 accelerated the removal of tebuconazole from soil.•Biochar-immobilized strain WZ-2 restored soil health by improving microbial activities and community composition. Tebuconazole is a widely used fungicide that may impair soil health. Presently, limited information is available on the bioremediation of tebuconazole-contaminated soil using biochar as a carrier for bacteria. In this study, we firstly isolated a tebuconazole-degrading strain and identified it as Alcaligenes faecalis WZ-2. Then, we used wheat straw-derived biochar as carrier to capture strain WZ-2 to assemble microorganism-immobilized composite. Finally, we investigated the effects of strain WZ-2 and biochar-immobilized WZ-2 on tebuconazole biodegradation, microbial enzyme activities and community composition in the contaminated soil. Results showed that, as compared to control, the strain WZ-2 and biochar-immobilized WZ-2 accelerated the degradation of tebuconazole, while reducing the half-life of tebuconazole from 40.8 to 18.7 and 13.3 days in soil, respectively. However, biochar alone than control slightly retarded the degradation of tebuconazole in soil. Though tebuconazole (10 mg/kg) negatively affected the soil enzyme activities (urease, dehydrogenase, and invertase) and microbiome community structure, the biochar-immobilized WZ-2 not only accelerated the degradation of tebuconazole but also restored native soil microbial enzyme activities and microbiome community composition. Our results suggest that a compatible combination of bacteria with biochar is an attractive and efficient approach for remediation of pesticide-contaminated soil and improvement of soil biological health.