A vinylpyrrolidone-based thin film microextraction in combination with direct solid-state spectrofluorimetry for determination of sartans in human plasma

• Published in: Analytica chimica acta 2020-08, Vol.1124, p.146-155
• Main Authors: Ghamat, Sima Najafi, Talebpour, Zahra
• Format: Article
• Language: English
• Subjects: Acrylates - chemistry; Angiotensin II Type 1 Receptor Blockers - blood; Ethylene glycol dimethacrylate; High-throughput extraction; Humans; Methacrylates - chemistry; Particle Size; Pyrrolidinones - chemistry; Sample preparation; Solid Phase Microextraction; Solid-state spectrofluorimetry; Spectrometry, Fluorescence; Surface Properties; Thin film microextraction; Vinylpyrrolidone
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2020.04.059

A vinylpyrrolidone-ethylene glycol dimethacrylate-acrylic acid thin film was prepared on a polypropylene guard and its formulation was optimized for application in thin film microextraction followed by direct solid-state spectrofluorimetry method. The surface morphology, fluorescence property and extraction performance of the thin film were investigated systematically. The intra- and inter-batch reproducibilities of thin film fabrication were obtained 2.3 and 4.2%, respectively. The lifetime of each prepared thin film was 30 times with a relative standard deviation of less than 1.4%. The developed method was optimized for extraction of some sartans as angiotensin II receptors antagonist (including losartan, valsartan, and olmesartan) which have been used to control hypertension as the main causes of cardiovascular disease. The optimum extraction conditions achieved at 2- (for losartan) and 4- (for valsartan and olmesartan) sample pH, 500-rpm rotation rate and 30-min extraction time for all three analytes. At the optimum conditions, analyses of losartan, valsartan, and olmesartan were validated in the human plasma matrix. Broad linearity ranges with determination coefficients of more than 0.999 were achieved for each calibration curve. Limit of detection of the method was 0.5 ng mL−1 for all three analytes. The intra- and inter-day accuracies and precisions of the developed method were evaluated in spiked plasma samples at three concentration levels of each analyte with high recoveries of 95–101% and relative standard deviations less than 6%. This method provides a simple, sensitive, fast, and high-throughput analysis method with the possibility of effective extraction of at least 40 samples simultaneously without the necessity of protein precipitating, desorption, and solvent evaporation steps. [Display omitted] •Vinylpyrrolidone-ethylene glycol dimethacrylate-acrylic acid was fabricated for TFME.•The thin film fabrication reproducibility and lifetime were suitable.•The advantages of TFME technique and solid-state fluorimetry were integrated.•The TFME-SSF method was successfully applied for analysis of some sartans in plasma.•Protein precipitation, desorption, and solvent evaporation steps were eliminated.