Hypercoagulability (thrombin generation) in patients with cirrhosis is detected with ST‐Genesia

• Published in: Journal of thrombosis and haemostasis 2020-09, Vol.18 (9), p.2177-2190
• Main Authors: Talon, Laurie, Sinegre, Thomas, Lecompte, Thomas, Pereira, Bruno, Massoulié, Sylvie, Abergel, Armand, Lebreton, Aurélien
• Format: Article
• Language: English
• Subjects: Automation; blood coagulation; blood coagulation test; Blood Coagulation Tests; Cirrhosis; clinical laboratory techniques; Humans; Liver cirrhosis; Liver Cirrhosis - complications; Liver Cirrhosis - diagnosis; Plasma; Reagents; Thrombin; Thrombomodulin; Thrombophilia - diagnosis
• ISSN: 1538-7933; 1538-7836; 1538-7836
• DOI: 10.1111/jth.14963

Background Thrombin generation assays (TGAs) performed with calibrated automated thrombography (CAT) in the presence of thrombomodulin (TM) indicate plasma hypercoagulability in cirrhosis. Objective To evaluate, in the presence of TM, the new ST‐Genesia automated device developed for improving TGA vs the previously used CAT method, with plasma samples of patients with cirrhosis. Patients/Methods Platelet‐poor plasma samples were prepared from citrated blood samples of 52 healthy controls and 85 patients with cirrhosis (severity evaluated using the Child‐Pugh score [CP]). TGAs were performed using CAT with PPP‐Reagent and ST‐Genesia with the STG‐ThromboScreen reagent, in the presence of TM. Endogenous thrombin potential (ETP) was chosen as the main parameter. Results Whatever the method, ETP values were higher in patients than in healthy controls. All patients identified as hypercoagulable with ST‐Genesia and STG‐ThromboScreen were found hypercoagulable with CAT and PPP‐Reagent. Conversely, eight and ten patients in the CP‐A and CP‐B classes respectively were identified as hypercoagulable only with CAT. The use of ST‐Genesia with the STG‐ThromboScreen reagent with TM led to a bias, with higher ETP values for healthy controls and lower for patients compared with CAT. Crossover analysis (CAT with the STG‐ThromboScreen reagent) evidenced a substantial effect of the STG‐ThromboScreen reagent; the analyzer (including calibration and data analysis) plays a lesser role. Conclusion ST‐Genesia evidences hypercoagulability in patients with cirrhosis when TG is studied in the presence of TM, but the results are not interchangeable with those obtained with CAT.