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Glucose effect on Candida albicans biofilm during tissue invasion
•Diabetic patients have higher salivary glucose levels than healthy ones.•The higher rates of salivary glucose are independent of whether plasmatic glucose is controlled or not.•Diabetic patients have more oral candidiasis than non-diabetic patients.•It is not clear if higher glucose levels could in...
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Published in: | Archives of oral biology 2020-09, Vol.117, p.104728-104728, Article 104728 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | •Diabetic patients have higher salivary glucose levels than healthy ones.•The higher rates of salivary glucose are independent of whether plasmatic glucose is controlled or not.•Diabetic patients have more oral candidiasis than non-diabetic patients.•It is not clear if higher glucose levels could interfere in Candida spp. invasion of oral tissue.
To evaluate, in vitro, the effect of two glucose concentrations (0.1 mM and 1.0 mM, simulating glucose concentration in saliva of healthy and diabetic individuals) on Candida albicans biofilm grown on epithelial monolayer.
C. albicans was inoculated on epithelial monolayers supplemented with 0.1 mM, 1.0 mM or no glucose. Control groups without C. albicans were also evaluated. Tissue response was assessed through the production of Interleukin-1α, Interleukin-8, Interleukin-6, Interleukin-10 and tumor necrosis factor-α. The complex of monolayer and biofilms were evaluated by quantitative reverse transcription polymerase chain reaction for expression of E-cadherin (CDH1), Caspase-3 (CASP3), β-defensin-1 (DEFB-1) and β-defensin-3 (DEFB-3). The biofilm architecture was visualized by confocal laser scanning microscopy.
The production of Interleukin-1α and Interleukin-8 were increased in the presence of C. albicans (p |
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ISSN: | 0003-9969 1879-1506 |
DOI: | 10.1016/j.archoralbio.2020.104728 |