Flavihumibacter soli sp. nov., Isolated from Soil

A novel Gram-stain-positive, strictly aerobic, non-motile, rod-shaped bacterium, designated strain R14T was isolated from steep grove soil and taxonomically characterized using a polyphasic approach. Cells showed oxidase-negative and catalase-positive activities and grew at 25–37 °C (optimum, 30 °C)...

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Published in:Current microbiology 2020-10, Vol.77 (10), p.3179-3184
Main Authors: Seo, Ye Lin, Jung, Jaejoon, Khan, Shehzad Abid, Kim, Kyung Hyun, Jeon, Che Ok
Format: Article
Language:English
Subjects:
Bacterial Typing Techniques
Bacteroidetes
Biomedical and Life Sciences
Biotechnology
Catalase
DNA, Bacterial - genetics
Fatty Acids
Gene sequencing
Genomes
Life Sciences
Lipids
Menaquinones
Microbiology
New species
Nucleotide sequence
pH effects
Phosphatidylethanolamine
Phylogenetics
Phylogeny
Quinones
RNA, Ribosomal, 16S - genetics
rRNA 16S
Sequence Analysis, DNA
Sodium chloride
Soil
Soil Microbiology
Soils
Vitamin K 2
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description A novel Gram-stain-positive, strictly aerobic, non-motile, rod-shaped bacterium, designated strain R14T was isolated from steep grove soil and taxonomically characterized using a polyphasic approach. Cells showed oxidase-negative and catalase-positive activities and grew at 25–37 °C (optimum, 30 °C), pH 5.0–8.0 (optimum, pH 6.5), and in the presence of 0–0.5% (w/v) NaCl (optimum, 0%). Phylogenetic trees based on 16S rRNA gene sequences revealed that strain R14T formed a phylogenetic lineage with Flavihumibacter genus members within the family Chitinophagaceae. Comparison of 16S rRNA gene sequences showed that strain R14T shared highest similarities with Flavihumibacter solisilvae 3-3T (97.5%), Flavihumibacter petaseus NBRC 106054T (96.7%), and Flavihumibacter stibioxidans YS-17T (96.1%). The G+C content of strain R14T calculated from its whole genome sequence was 44.6 mol%. Strain R14T contained menaquinone-7 and iso-C 15:0 , iso-C 17:0 3-OH, C 16:0 , C 15:1 ω6c, and iso-C 15:0 -G as the sole respiratory isoprenoid quinone and major cellular fatty acids, respectively. Major polar lipids consisted of phosphatidylethanolamine, two unidentified aminolipids, and four unidentified lipids. Based on phylogenetic, phenotypic, and chemotaxonomic characteristics of strain R14T, it could be concluded that strain R14T represents a novel species of the genus Flavihumibacter , for which the name Flavihumibacter soli sp. nov. is proposed. The type strain is R14T (=KACC 21229T = JCM 33473T).
doi_str_mv 10.1007/s00284-020-02103-2
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Major polar lipids consisted of phosphatidylethanolamine, two unidentified aminolipids, and four unidentified lipids. Based on phylogenetic, phenotypic, and chemotaxonomic characteristics of strain R14T, it could be concluded that strain R14T represents a novel species of the genus Flavihumibacter , for which the name Flavihumibacter soli sp. nov. is proposed. 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source Springer Nature
subjects Bacterial Typing Techniques
Bacteroidetes
Biomedical and Life Sciences
Biotechnology
Catalase
DNA, Bacterial - genetics
Fatty Acids
Gene sequencing
Genomes
Life Sciences
Lipids
Menaquinones
Microbiology
New species
Nucleotide sequence
pH effects
Phosphatidylethanolamine
Phylogenetics
Phylogeny
Quinones
RNA, Ribosomal, 16S - genetics
rRNA 16S
Sequence Analysis, DNA
Sodium chloride
Soil
Soil Microbiology
Soils
Vitamin K 2
title Flavihumibacter soli sp. nov., Isolated from Soil
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