Genome‐wide comparison of Carnobacterium maltaromaticum derived from diseased fish harbouring important virulence‐related genes

Although Carnobacterium maltaromaticum has been used as a probiotic in fish, it was reported to cause disease for the first time in Korea. The objective of this study was to understand the differences between pathogenic and non‐pathogenic strains. Pathogenicity was tested by challenging rainbow trou...

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Bibliographic Details
Published in:Journal of fish diseases 2020-09, Vol.43 (9), p.1029-1037
Main Authors: Roh, HyeongJin, Kim, Bo Seong, Lee, Mu Kun, Park, Chan‐Il, Kim, Do‐Hyung
Format: Article
Language:English
Subjects:
Animals
Aquaculture
Blood cells
Carnobacterium - genetics
Carnobacterium - pathogenicity
Carnobacterium maltaromaticum
Dairy products
Fish
Fish diseases
Fish Diseases - microbiology
Fish oils
Freshwater fishes
Genes
Genome, Bacterial
Genomes
genome‐wide comparison
Gram-Positive Bacterial Infections - microbiology
Gram-Positive Bacterial Infections - veterinary
Histopathology
Oncorhynchus mykiss
Pathogenicity
Pathogens
Probiotics
rainbow trout
Republic of Korea
Teichuronic acid
Trout
Virulence
Virulence - genetics
Virulence factors
wecC
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Summary:Although Carnobacterium maltaromaticum has been used as a probiotic in fish, it was reported to cause disease for the first time in Korea. The objective of this study was to understand the differences between pathogenic and non‐pathogenic strains. Pathogenicity was tested by challenging rainbow trout with C. maltaromaticum ATCC35586 and 18ISCm isolated from diseased fish, and DSM20342 isolated from a dairy product. We also compared 24 genomes of C. maltaromaticum strains plus the genome of our isolate 18ISCm sequenced in this study. Only the strains from diseased fish caused high mortality with severe histopathological changes. Although all strains shared more than 90% of Ko_id, wecC and xtmA were found only in strains from diseased fish. Interestingly, only strains from diseased fish harboured two wecC paralogs involved in the production of D‐mannosaminuronic acid which is a major component of a well‐known virulence factor, teichuronic acid. Two wecC paralogs of 18ISCm were increased when they were co‐cultured with trout blood cells, suggesting that wecC genes might play a role in virulence. The results of this study show that strains isolated from diseased fish are different from strains derived from food in terms of pathogenicity to fish and the presence of virulence‐related genes.
ISSN:0140-7775
1365-2761
DOI:10.1111/jfd.13208