Hsp90 interacts with Cdc37, is phosphorylated by PKA/PKC, and regulates Src phosphorylation in human sperm capacitation

Background Heat shock protein 90 (Hsp90) signaling pathways participate in protein phosphorylation during sperm capacitation. However, the underlying mechanism is largely unknown. Objective The aim of this study was to explore the interaction between Hsp90 and its co‐chaperone protein, cell division...

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Published in:Andrology (Oxford) 2021-01, Vol.9 (1), p.185-195
Main Authors: Li, Kun, Sun, Peibei, Wang, Yayan, Gao, Tian, Zheng, Dongwang, Liu, Ajuan, Ni, Ya
Format: Article
Language:English
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Summary:Background Heat shock protein 90 (Hsp90) signaling pathways participate in protein phosphorylation during sperm capacitation. However, the underlying mechanism is largely unknown. Objective The aim of this study was to explore the interaction between Hsp90 and its co‐chaperone protein, cell division cycle protein Cdc37 (Cdc37), in human spermatozoa. Materials and methods We examined the effects of H‐89 (a protein kinase A [PKA] inhibitor) and Go6983 (a protein kinase C [PKC] inhibitor) on the phosphorylation of serine, threonine, and tyrosine residues in Hsp90; the effect of 17‐allylamino‐17‐demethoxygeldanamycin (17‐AAG, Hsp90 inhibitor) on Y416‐Src phosphorylation; and the effects of 17‐AAG and geldanamycin on threonine phosphorylation during human sperm capacitation. Results Hsp90 co‐localized and interacted with Cdc37. During human sperm capacitation, Hsp90 phosphorylation at serine, threonine, and tyrosine residues was inhibited by H‐89 and Go6983. In addition, phosphorylation of residue Y416 in the tyrosine kinase Src (its active site) was inhibited by 17‐AAG, and the threonine phosphorylation levels of some proteins were decreased by 17‐AAG and geldanamycin. Discussion and conclusion Taken together, our data showed that the interaction of Hsp90 with Cdc37 regulates total protein threonine phosphorylation and Src phosphorylation via its serine, threonine, and tyrosine phosphorylation, which are controlled by PKA and PKC during human sperm capacitation. The results of this study help understand the mechanism underlying Hsp90 regulation of sperm function.
ISSN:2047-2919
2047-2927
DOI:10.1111/andr.12862