Insights into cathepsin-B activity in mature dentin matrix

•Why Cathepsins co-exist with MMPs in mature dentin matrix is presently unknown.•Leading activity of one group of protease over other is dependent on pH.•Along with MMPs, cathepsin B can exert proteolysis of dentin matrix at neutral pH. Cysteine proteases are lysosomal enzymes that, under specific c...

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Published in:Archives of oral biology 2020-09, Vol.117, p.104830-104830, Article 104830
Main Authors: Carrilho, Marcela R., Scaffa, Polliana, Oliveira, Vitor, Tjäderhane, Leo, Tersariol, Ivarne L., Pashley, David H., Tay, Franklin, Nascimento, Fabio D.
Format: Article
Language:English
Subjects:
Cathepsins
Collagen
Cysteine proteases
Degradation
Dentin
Dentistry
Proteolytic activity
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Summary:•Why Cathepsins co-exist with MMPs in mature dentin matrix is presently unknown.•Leading activity of one group of protease over other is dependent on pH.•Along with MMPs, cathepsin B can exert proteolysis of dentin matrix at neutral pH. Cysteine proteases are lysosomal enzymes that, under specific circumstances, may be secreted into the extracellular space and participate in protein turnover. This study investigated the involvement of cathepsin B in the gelatinolytic activity of mature dentin matrices at neutral pH. Human dentin fragments were made into powder and enzymes were extracted using guanidine-HCl/EDTA. Host-derived dentin proteases (cathepsin B, MMP-2 and MMP-9) were identified by immunoblotting, and their activities were evaluated spectrofluorimetrically using fluorogenic substrates. Proteases activities were monitored by measuring the rate of hydrolysis of substrates in the presence/absence of MMP- or cysteine cathepsin inhibitors, at neutral pH (7.4). Mass spectroscopy was used to determine the substrates’ cleavage points. Reverse zymography was performed to examine the gelatinolytic activity of cathepsin B. Western-blots of dentin extracts yielded strong bands at 95, 72 and 30 kDa, corresponding respectively to MMP-9, MMP-2 and Cathepsin B. Greater fluorogenic substrates hydrolysis occurred in the absence of MMP and cysteine cathepsin inhibitors than in their presence. Cathepsin B exhibited significant gelatinolytic activity. Together with MMP-2 and MMP-9, cathepsin B also account for the host-derived gelatinolytic activity and matrix turnover of mature dentin at physiological, neutral pH.
ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2020.104830