A nanocomposite consisting of cuprous oxide supported on graphitic carbon nitride nanosheets for non-enzymatic electrochemical sensing of 8-hydroxy-2′-deoxyguanosine

• Published in: Mikrochimica acta (1966) 2020-08, Vol.187 (8), p.459-459, Article 459
• Main Authors: Rajaji, Umamaheswari, Selvi, Subash Vetri, Chen, Shen-Ming, Chinnapaiyan, Sathishkumar, Chen, Tse-Wei, Govindasamy, Mani
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Biological properties; Biomarkers; Boron nitride; Carbon; Carbon nitride; Characterization and Evaluation of Materials; Charge transfer; Chemical sensors; Chemistry; Chemistry and Materials Science; Copper oxide; Copper oxides; Cubes; Cuprite; Electric properties; Electrical measurement; Electrode materials; Electrodes; Electron transfer; Enzymes; Microengineering; Nanochemistry; Nanocomposites; Nanosheets; Nanotechnology; Original Paper; Oxidative stress; Phosphates; Reproducibility; Selectivity; Sensors
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-020-04416-2

Graphitic carbon nitrides supported cuprous oxide architecture is reported as an efficient electrode material for supercapacitors, especially due to its high charge-transfer conductivity of the electrochemical devices. Herein, we present an electrochemical sensor to specifically detect 8-hydroxy-2′-deoxyguanosine (8-HDG) oxidative stress biomarker using graphitic carbon nitrides that decorate a cuprous oxide cubes modified electrode. The fabricated electrochemical sensor was characterized and proved by electrochemical methods, EDX, FESEM, and amperometry (i–t). In the presence of 8-hydroxy-2′-deoxyguanosine (8-HDG), the effective interaction between graphitic carbon nitrides and 8-HDG favors the accumulation on the Cu 2 O/g-C 3 N 4 /GCE, which increases the electrocatalytic property and amperometric response. The proposed electrochemical sensor exhibits a wide linear range for 8-HDG in 0.1 M phosphate buffer (pH 7.0) from 25 nM to 0.91 mM, and the limit of detection (LOD) is 4.5 nM. The stability of the Cu 2 O/g-C 3 N 4 /GCE is improved when stored at 4 °C. The repeatability and reproducibility of this electrochemical sensor is good and the sensor retains its  current response for 8-HDG detection also after long time storage. The modified sensor proved high selectivity and sensitivity for 8-HDG, which made it possible to determine 8-HDG in biological samples. Furthermore, the Cu 2 O/g-C 3 N 4 /GCE offered a favorable electron transfer between the Cu 2 O/g-C 3 N 4 and the electrode interface compared to Cu 2 O/GCE, g-C 3 N 4 /GCE, and unmodified GCE. Graphical abstract Electrochemical detection of oxidative stress marker based on Cu 2 O@g-C 3 N 4 materials modified electrode.