Collaborative study: Quantification of total folate in food using an efficient single-enzyme extraction combined with LC-MS/MS

•Specific LC-MS/MS method for folate analysis in food is inter-laboratory validated.•Total folate results acceptable in six tested food samples; for salmon unacceptable.•HorRat values for total folate and 5-CH3-H4folate were within the acceptable range.•Acceptability of HorRat values for other studi...

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Published in:Food chemistry 2020-12, Vol.333, p.127447-127447, Article 127447
Main Authors: Ložnjak Švarc, Petra, Oveland, Eystein, Strandler, Hanna Sara, Kariluoto, Susanna, Campos-Giménez, Esther, Ivarsen, Elise, Malaviole, Isabelle, Motta, Carla, Rychlik, Michael, Striegel, Lisa, Jakobsen, Jette
Format: Article
Language:English
Subjects:
Deconjugase
Homogenization
Inter-laboratory study
Plant enzyme
Tetrahydrofolates
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Summary:•Specific LC-MS/MS method for folate analysis in food is inter-laboratory validated.•Total folate results acceptable in six tested food samples; for salmon unacceptable.•HorRat values for total folate and 5-CH3-H4folate were within the acceptable range.•Acceptability of HorRat values for other studied vitamers depends on food matrix.•For total folate: repeatability at 2.3%–15% and reproducibility at 8.7%–58% Quantification of the specific folate vitamers to estimate total folate in foods is not standardized. A collaborative study, including eight European laboratories, was conducted in order to determine the repeatability and reproducibility of the method for folate quantification in foods using the plant-origin γ-glutamyl hydrolase as part of the extraction procedure. The seven food samples analyzed represent the food groups; fruits, vegetables, dairy products, legumes, offal, fish, and fortified infant formula. The homogenization step was included, and six folate vitamers were analyzed using LC-MS/MS. Total folate content, expressed as folic acid equivalent, was 17–490 μg/100 g in all samples. Horwitz ratio values were within the acceptable range (0.60–1.94), except for fish. The results for fortified infant formula, a certified reference material (NIST 1869), confirmed the trueness of the method. The collaborative study is part of a standardization project within the Nordic Committee on Food Analysis (NMKL).
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2020.127447