Novel culture system via wirelessly controllable optical stimulation of the FGF signaling pathway for human and pig pluripotency

Stem cell fate is largely determined by cellular signaling networks and is heavily dependent on the supplementation of exogenous recombinant proteins into culture media; however, uneven distribution and inconsistent stability of recombinant proteins are closely associated with the spontaneous differ...

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Bibliographic Details
Published in:Biomaterials 2021-02, Vol.269, p.120222-120222, Article 120222
Main Authors: Choi, In Young, Lim, HoTae, Huynh, Alex, Schofield, James, Cho, Hyeon Jin, Lee, Hosuk, Andersen, Peter, Shin, Joo Heon, Heo, Won Do, Hyun, Sang-Hwan, Kim, Yong Jun, Oh, Yohan, Kim, Hyesoo, Lee, Gabsang
Format: Article
Language:English
Subjects:
FGF signaling
FGF2
Optogenetics
Pluripotency
Pluripotent stem cell
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Summary:Stem cell fate is largely determined by cellular signaling networks and is heavily dependent on the supplementation of exogenous recombinant proteins into culture media; however, uneven distribution and inconsistent stability of recombinant proteins are closely associated with the spontaneous differentiation of pluripotent stem cells (PSCs) and result in significant costs in large-scale manufacturing. Here, we report a novel PSC culture system via wirelessly controllable optical activation of the fibroblast growth factor (FGF) signaling pathway without the need for supplementation of recombinant FGF2 protein, a key molecule for maintaining pluripotency of PSCs. Using a fusion protein between the cytoplasmic region of the FGF receptor-1 and a light-oxygen-voltage domain, we achieved tunable, blue light-dependent activation of FGF signaling in human and porcine PSCs. Our data demonstrate that a highly controllable optical stimulation of the FGF signaling pathway is sufficient for long-term maintenance of PSCs, without the loss of differentiation potential into three germ layers. This culture system will be a cost-effective platform for a large-scale stem cell culture.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2020.120222