Paper-based electrochemiluminescence determination of streptavidin using reticular DNA-functionalized PtCu nanoframes and analyte-triggered DNA walker

A paper-based electrochemiluminescence (ECL) biosensor characterized by the signal amplification of reticular DNA-functionalized PtCu nanoframes (DNA-PtCuTNFs) and analyte-triggered DNA walker was developed for sensitive streptavidin assay. Silver microflower functionalized paper-based sensing platf...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2020-09, Vol.187 (9), p.530-530, Article 530
Main Authors: Huang, Yuzhen, Zhang, Lina, Zhang, Sibao, Zhao, Peini, Li, Li, Ge, Shenguang, Yu, Jinghua
Format: Article
Language:English
Subjects:
Analytical Chemistry
Biosensors
Biotin
Catalysis
Characterization and Evaluation of Materials
Chemical synthesis
Chemistry
Chemistry and Materials Science
Deoxyribonucleic acid
Detectors
DNA
Electrochemiluminescence
Intermetallic compounds
Microengineering
Nanochemistry
Nanotechnology
Original Paper
Peroxidase
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Summary:A paper-based electrochemiluminescence (ECL) biosensor characterized by the signal amplification of reticular DNA-functionalized PtCu nanoframes (DNA-PtCuTNFs) and analyte-triggered DNA walker was developed for sensitive streptavidin assay. Silver microflower functionalized paper-based sensing platform was prepared to fix the hairpin strand (S1). With addition of the streptavidin, plenty of DNA walkers consisting of the walking strands (S2) labeled with biotin and streptavidin were established, which protected S2 from digestion via the terminal protection mechanism. The sequential introduction of the DNA walker and capture probe initiated the hairpin structure opening of S1 and strand displacement reaction (SDR) happening, causing the S2 release. Subsequently, S1 hybridized with S3. The free S2 further hybridized with adjacent S1 to trigger the next cycle. After multiple cycles, the DNA-PtCuTNFs, the fire-new signal enhancer, with remarkable peroxidase activity, were successfully attached onto the paper electrode via metal-catalyst-free click chemistry. Based on the SDR of the DNA walker and the catalysis of DNA-PtCuTNFs, a significantly boosted ECL signal of luminol was obtained. Under the optimal conditions, the developed sensor for streptavidin assay exhibited a low detection limit of 33.4 fM with a linear range from 0.1 pM to 0.1 μM. Graphical abstract
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-020-04515-0