Development of the smooth muscle layer in the ileum of mouse embryos

The smooth muscle layer (SML) comprises a significant portion of the intestines and other tubular organs. Whereas epithelial development has recently been extensively studied, SML development has drawn relatively less attention. Previous morphological reports revealed that the inner circular layer (...

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Bibliographic Details
Published in:Anatomical science international 2021, Vol.96 (1), p.97-105
Main Authors: Jahan, Esrat, Rafiq, Ashiq Mahmood, Matsumoto, Akihiro, Jahan, Nusrat, Otani, Hiroki
Format: Article
Language:English
Subjects:
Anatomy
Animal Anatomy
Animal Physiology
Animals
Cell Biology
Cell Differentiation
Embryos
Histology
Human Physiology
Ileum
Ileum - cytology
Ileum - embryology
Intestine
Medicine
Medicine & Public Health
Mesentery
Mice
Mice, Inbred C57BL
Morphology
Muscle, Smooth - cytology
Muscle, Smooth - embryology
Myocytes, Smooth Muscle - physiology
Neurosciences
Organogenesis
Organogenesis - physiology
Original Article
Smooth muscle
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Summary:The smooth muscle layer (SML) comprises a significant portion of the intestines and other tubular organs. Whereas epithelial development has recently been extensively studied, SML development has drawn relatively less attention. Previous morphological reports revealed that the inner circular layer (IC) differentiates earlier than the outer longitudinal layer (OL), but detailed development of the SML, including chronological changes in the cell layer number, precise cell orientation, and regional differences in relation to the mesentery, has not been reported. We here observed the development of the SML in the C57BL/6J mouse ileum near the ileocecal junction at embryonic day (E) 13.5, 15.5, and 17.5. By histo-morphometric analyses, in IC, smooth muscle cells (SMCs) were oval-shaped and irregularly arranged in 3–4 layers at E13.5, then adopted an elongated spindle shape and decreased to two cell layers at E15.5 and E17.5. The IC SMC nuclear angle was not vertical, but oriented at 60–80° against the mid-axis of the intestinal lumen. The single SMC layer in OL was observed at E17.5, and the SMC nuclear angle was parallel to the luminal mid-axis. No clear regional difference against the mesentery was observed. Collectively, the findings suggest that development and differentiation of the ileal SML is not simple but regulated in a complex manner and possibly related to the macroscopic organogenesis.
ISSN:1447-6959
1447-073X
DOI:10.1007/s12565-020-00565-9