Static Magnetic Stimulation Induces Cell-type Specific Alterations in the Viability of SH-SY5Y Neuroblastoma Cell Line

Background/Aim: Magnetic stimulation is used in the treatment of a diversity of diseases, but a complete understanding of the underlying mechanisms of action requires further investigation. We examined the effect of static magnetic stimulation (SMS) in different cell lines. Materials and Methods: A...

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Bibliographic Details
Published in:Anticancer research 2020-09, Vol.40 (9), p.5151-5158
Main Authors: MEDEIROS, HELOUISE R., ASSUMPCAO, JOSÉ A. F., MEDEIROS, LICIANE F., STAPENHORST, MARTINA, NUNES, LARA, HENCKES, NICOLE A.C., CRUZ, CAROLINA URIBE, FREGNI, FELIPE, SANCHES, PAULO R.S., OLIVEIRA, FERNANDA S.O., CAUMO, WOLNEI, CIRNE-LIMA, ELIZABETH O., TORRES, IRACI L.S.
Format: Article
Language:English
Subjects:
Apoptosis
Biotechnology
Brain-derived neurotrophic factor
Cell culture
Cell cycle
Cell death
Cell lines
Cell viability
Magnetic fields
Magnets
Medical treatment
Short message service
Stimulation
Tumors
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Summary:Background/Aim: Magnetic stimulation is used in the treatment of a diversity of diseases, but a complete understanding of the underlying mechanisms of action requires further investigation. We examined the effect of static magnetic stimulation (SMS) in different cell lines. Materials and Methods: A culture plate holder with attached NeFeB magnets was developed. Different magnetic field intensities and periods were tested in tumoral and non-tumoral cell lines. To verify the cellular responses to SMS, cell viability, cell death, cell cycle and BDNF expression were evaluated. Results: Exposure of SH-SY5Y cells to SMS for 24 hours led to a decrease in cell viability. Analysis 24 h after stimulation revealed a decrease in apoptotic and double-positive cells, associated with an increase in the number of necrotic cells. Conclusion: The effects of SMS on cell viability are cell type-specific, inducing a decrease in cell viability in SH-SY5Y cells. This suggests that SMS may be a potential tool in the treatment of neuronal tumors.
ISSN:0250-7005
1791-7530
DOI:10.21873/anticanres.14518